HYPOTHALAMIC VARIATION--SEX, SEXUAL ORIENTATION & AIDS

下丘脑变异——性别、性取向

• 批准号: 2034410
• 负责人: WILLIAM M BYNE
• 金额: $ 7.44万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2034410
• 关键词: AIDS; behavioral /social science research tag; brain mapping; gender difference; histology; homosexuals; human tissue; hypothalamus; immunocytochemistry; in situ hybridization; interstitial; laboratory rat; phenotype; postmortem; sex behavior; thalamic nuclei

The proposed research will examine the interstitial nuclei of the human anterior hypothalamus (INAH) for volumetric variation associated with infection with the human immunodeficiency virus in an experimental design that assesses and controls for variation associated with age, gender category, and various sequelae of infection. The cellular parameters (i.e., cell size and number) that give rise to any such confirmed volumetric variation will be determined. A detailed analysis of the cellular phenotypes that comprise the INAH will be done using immunocytochemistry and in situ hybridization to localize a variety of peptides, enzymes and putative neurotransmitters. The significance of the proposed research is that it will provide the first detailed analysis of the cellular phenotypes of the human INAH. The proposed research may: resolve discrepancies among laboratories regarding hormonal influences on brain structure developmentally and in adulthood, suggest ways in which HIV influences brain structure, facilitate functional studies by suggesting homologies between specific human INAH and specific hypothalamic nuclei of laboratory animals. In addition to raising interesting questions about the mechanisms linking the structure of the brain to its function, hypothalamic variations in animals have provided powerful models for studying the cellular and molecular aspects of neural development and its hormonal regulation. Confirmation of similar variations in the human hypothalamus could similarly provide insights into important neurodevelopmental mechanisms of potentially broad clinical significance.

这项拟议的研究将检查人类的间质核。 下丘脑前部(INAH)的体积变异与 实验设计中的人类免疫缺陷病毒感染 评估和控制与年龄、性别相关的变异 类别，以及各种感染后遗症。蜂窝参数 (即单元格大小和数量)会导致任何此类确认 体积变化将被确定。详细分析了 组成异烟肼的细胞表型将使用 免疫细胞化学和原位杂交定位多种肿瘤细胞 多肽、酶和可能的神经递质。这一事件的意义 拟议的研究将提供第一个详细的分析 人类INAH的细胞表型。拟议的研究可能： 解决实验室之间关于荷尔蒙影响的差异 大脑结构在发育和成年期，建议的方式 艾滋病毒通过以下方式影响大脑结构，促进功能研究 提示特定人类INAH和特定INAH之间的同源性 实验动物的下丘脑核团。除了筹集资金 关于链接结构的机制的有趣问题 大脑对其功能、下丘脑的变异都为动物提供了 研究神经细胞和分子方面的强大模型 发育及其荷尔蒙调节。类似产品的确认 人类下丘脑的变化同样可以提供对 潜在临床应用的重要神经发育机制 意义。