NEURONAL DEVELOPMENT IN THE WING OF THE FRUITFLY

果蝇翅膀的神经元发育

• 批准号: 2445769
• 负责人: SETH S BLAIR
• 金额: $ 16.2万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2445769
• 关键词: Drosophilidae; developmental genetics; gene expression; genetic manipulation; immunocytochemistry; neurogenesis; neurogenetics; peripheral nervous system; transcription factor

DESCRIPTION: This proposal outlines a series of experiments designed to test and refine our understanding of the mechanisms by which the developing nervous system is precisely patterned. Such precision is essential to the normal functioning of the brain; the means by which such patterning arises is essential to both our understanding of normal fetal development, and for developing therapies for the recovery from disease and injury-induced trauma to the nervous system. The ability of researchers to experimentally analyze brain development in vertebrates, however, is limited by the complexity of the vertebrate nervous system and difficulties making appropriate experimental manipulations. This proposal therefore outlines an ongoing series of experiments which examine the patterned development of the peripheral nervous system in the wing of the fruitfly, Drosophila melanogaster. In the fruitfly wing, the individual elements of the PNS arise in an extremely stereotyped fashion from a single epithelial sheet. The relative simplicity of the system and the availability of a number of molecular and genetic tools make it very favorable for experimental analyses. We will use a combination of genetic, molecular, and immunohistological techniques to analyze the molecular basis of this stereotypy, concentrating especially on the sensory organs that arise along the anterior wing margin. Our analysis includes testing the roles of a number of known genes and cell signalling pathways, and continuing a search for novel genes critical to the patterning process. Our specific aims are to: 1. Examine the mechanisms underlying the formation of the dorsoventral compartment boundary. 2. Examine the role of the apterous boundary in localizing margin-specific gene expression; 3. Test the role of the margin-specific scalloped transcription factor in margin formation. 4. Test the role of the Notch signalling pathway in inducing margin wingless expression and neuronal development; 5. Examine the mechanisms by which wg can refine its domain of expression. 6) Examine the development and refinement of neural regions; 7) Screen for and characterize novel patterning genes using enhancer traps and the FLP-FRT Fl screening method.

描述：这项提案概述了一系列旨在 测试和完善我们对发育的机制的理解 神经系统被精确地构筑成图案。这样的精确度对于 大脑的正常运作；产生这种模式的方式 对我们理解正常的胎儿发育和 发展从疾病和创伤中恢复的治疗方法 对神经系统的影响。研究人员进行实验分析的能力 然而，脊椎动物的大脑发育受到大脑发育的复杂性的限制。 脊椎动物的神经系统和使适当的 实验性的操控。因此，该提案概述了正在进行的 一系列的实验，考察了 果蝇翅膀的外周神经系统 黑猩猩。在果蝇的翅膀上，PNS的个体元素 以一种非常刻板的方式从一张上皮片上长出来。 系统的相对简单性和一些可用的 分子和基因工具使其非常有利于实验 分析。我们将使用遗传、分子和 免疫组织学技术分析这一疾病的分子基础 刻板印象的，尤指集中在沿途出现的感觉器官上的 前翼边缘。我们的分析包括测试一个 已知基因和细胞信号通路的数量，并继续搜索 寻找对构图过程至关重要的新基因。我们的具体目标是 TO：1.研究背腹侧部形成的机制 隔间边界。2.研究无翼边界在 定位边缘特异的基因表达；3.测试基因的作用 边缘特异的扇形转录因子在边缘形成中的作用。4. 检测Notch信号通路在诱导边缘无翅中的作用 表达与神经元发育；5.检测WG的机制 可以细化它的表达领域。6)检查发展和 神经区域的精细化；7)筛选和表征小说 使用增强子陷阱和FLP-FRT F1筛选方法构建基因图案化。