NEURONAL DEVELOPMENT IN THE WING OF THE FRUITFLY

果蝇翅膀的神经元发育

• 批准号: 2891766
• 负责人: SETH S BLAIR
• 金额: $ 17.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2891766
• 关键词: Drosophilidae; developmental genetics; gene expression; genetic manipulation; immunocytochemistry; neurogenesis; neurogenetics; peripheral nervous system; transcription factor

DESCRIPTION: This proposal outlines a series of experiments designed to test and refine our understanding of the mechanisms by which the developing nervous system is precisely patterned. Such precision is essential to the normal functioning of the brain; the means by which such patterning arises is essential to both our understanding of normal fetal development, and for developing therapies for the recovery from disease and injury-induced trauma to the nervous system. The ability of researchers to experimentally analyze brain development in vertebrates, however, is limited by the complexity of the vertebrate nervous system and difficulties making appropriate experimental manipulations. This proposal therefore outlines an ongoing series of experiments which examine the patterned development of the peripheral nervous system in the wing of the fruitfly, Drosophila melanogaster. In the fruitfly wing, the individual elements of the PNS arise in an extremely stereotyped fashion from a single epithelial sheet. The relative simplicity of the system and the availability of a number of molecular and genetic tools make it very favorable for experimental analyses. We will use a combination of genetic, molecular, and immunohistological techniques to analyze the molecular basis of this stereotypy, concentrating especially on the sensory organs that arise along the anterior wing margin. Our analysis includes testing the roles of a number of known genes and cell signalling pathways, and continuing a search for novel genes critical to the patterning process. Our specific aims are to: 1. Examine the mechanisms underlying the formation of the dorsoventral compartment boundary. 2. Examine the role of the apterous boundary in localizing margin-specific gene expression; 3. Test the role of the margin-specific scalloped transcription factor in margin formation. 4. Test the role of the Notch signalling pathway in inducing margin wingless expression and neuronal development; 5. Examine the mechanisms by which wg can refine its domain of expression. 6) Examine the development and refinement of neural regions; 7) Screen for and characterize novel patterning genes using enhancer traps and the FLP-FRT Fl screening method.

描述：该提案概述了一系列旨在 测试和完善我们的理解的机制， 神经系统的模式是精确的 这样的精确度对于 大脑的正常功能;这种模式产生的方式 对于我们理解正常的胎儿发育和 开发用于从疾病和损伤引起的创伤中恢复的疗法 到神经系统。 研究人员的实验分析能力 然而，脊椎动物的大脑发育受到以下复杂性的限制： 脊椎动物的神经系统和困难，使适当的 实验操作。 因此，本提案概述了一项正在进行的 一系列的实验，检查模式的发展， 果蝇翅膀的周围神经系统 黑腹菌 在果蝇的翅膀中，PNS的单个元件 以一种非常刻板的方式从单一的上皮层产生。 该系统相对简单，并提供了一些 分子和遗传工具使其非常有利于实验 分析。 我们将结合基因、分子和 免疫组织学技术来分析这种疾病的分子基础。 刻板，特别是集中在感觉器官出现沿着 前翅缘 我们的分析包括测试 一些已知的基因和细胞信号通路，并继续寻找 寻找对图案形成过程至关重要的新基因。 我们的具体目标是 至：1. 研究背腹侧的形成机制 隔室边界 2. 研究无翅边界在 定位边缘特异性基因表达; 3. 测试的作用 边缘形成中的边缘特异性扇形转录因子。 4. 测试Notch信号通路在诱导边缘无翼中的作用 表达和神经元发育; 5. 审查工作组 可以改善它的表达领域。 6)研究发展和 神经区域的细化; 7）筛选和表征新的 使用增强子陷阱和FLP-FRT F1筛选方法对基因进行模式化。