Axes and compartments in the wing of the fruitfly

果蝇翅膀上的轴和隔间

基本信息

批准号：
7204116
负责人：
SETH S BLAIR
金额：
$ 25.31万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-08-01 至 2011-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7204116
关键词：
Adhesives Affect Alleles Animals Anterior Binding Biochemical Biological Assay Cell Polarity Cells Cleaved cell Development Disease Distal Drosophila genus Drosophila melanogaster Epithelial Cells Erinaceidae Extracellular Domain Extracellular Matrix Extracellular Space Family Fatty acid glycerol esters Fetal Development Fetus Genes Genetic Genetic Epistasis Golgi Apparatus Grant Growth Heparan Sulfate Proteoglycan Homologous Gene Human Human Development Injury Joints Ligands Lipids Mediating Modification Molecular Molecular Genetics Movement Mutate Mutation Neurons Numbers Orthologous Gene Pathway interactions Pattern Phenotype Play Process Proteins Publishing Recovery Regulation Relative (related person)Research Personnel Role Series Signal Pathway Signal Transduction Signaling Pathway Gene Specific qualifier value Specificity Stereotyping Structure System Techniques Tertiary Protein Structure Testing Tissues Vertebrates Wing Wnt proteins Work appendage base cofactor design extracellular human CYP2B6 protein member mutant novel palmitoylation programs receptor research study smoothened signaling pathway tool vertebrate genome

项目摘要

DESCRIPTION (provided by applicant): This proposal outlines a series of experiments designed to test and refine our understanding of the mechanisms by which the developing tissues precisely patterned. Such precision is essential to normal human development, and analyzing the means by which such patterning arises, is essential to both our understanding of normal fetal development, and for developing therapies for the recovery from disease and injury. The ability of researchers to experimentally analyze development in vertebrates is limited, however, by the complexity of the vertebrate genome and fetus. This proposal therefore outlines an ongoing series of experiments that analyze these problems in the developing appendages of the fruitfly, Drosophila melanogaster. In the fruitfly wing, specific tissues arise in an extremely stereotyped fashion from a single layer of epithelial cells. Not only is this system simple, but the genetic and molecular tools available for work on Drosophila are unmatched by any other animal system. We will use a combination of genetic, histochemical and molecular techniques to analyze the developmental bases of the stereotyped neuronal development found in Drosophila. Our analyses include testing the roles of a number of known genes and signaling pathways, and searching for novel genes critical to the patterning process. Our first aim is to analyze the role of the secreted protein Shifted, a homolog of human Wnt Inhibitory Factor-1, and how it interacts with Hedgehog signaling during the induction of cells along compartment boundaries. Our second aim is examine a novel signaling mechanism by which two unusual protocadherins, Fat and Dachsous, regulate the growth and proximal-distal patterning.

描述（由申请人提供）：该提案概述了一系列实验，旨在测试和完善我们对开发组织精确图案的机制的理解。这种精度对于正常的人类发展至关重要，并且分析产生这种模式的手段对于我们对正常胎儿发育的理解以及开发从疾病和损伤中恢复的疗法至关重要。然而，研究人员实验分析脊椎动物的发育的能力受脊椎动物基因组和胎儿的复杂性有限。因此，该提案概述了正在进行的一系列实验，这些实验在果蝇果蝇的发展附属物中分析了这些问题。在果蝇翼中，特定的组织以一种非常刻板的方式来自单层上皮细胞。该系统不仅简单，而且可用于果蝇上工作的遗传和分子工具是任何其他动物系统都无法比拟的。我们将使用遗传，组织化学和分子技术的组合来分析果蝇中发现的刻板神经元发育的发育基础。我们的分析包括测试许多已知基因和信号通路的作用，以及寻找对模式过程至关重要的新基因。我们的第一个目的是分析分泌蛋白转移的作用，人类Wnt抑制因子1的同源物，以及在沿隔室边界诱导细胞期间与刺猬信号传导相互作用。我们的第二个目的是检查一种新型的信号传导机制，通过这种机制，通过这种机制，通过这种机制，通过这种机制，通过这种机制，脂肪和5a的含量，可以调节生长和近端模式。