DROSOPHILA GENES AFFECTING CHROMOSOME SEGREGATION

影响染色体分离的果蝇基因

• 批准号: 2022643
• 负责人: MICHAEL L GOLDBERG
• 金额: $ 25.71万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2022643
• 关键词: Caenorhabditis elegans; Drosophilidae; cell cycle; chromosome movement; cytogenetics; gene expression; gene interaction; immunoelectron microscopy; intracellular transport; laboratory rabbit; lasers; microtubules; protein structure function; protein transport; proteins; video microscopy

This proposal is focused on the genetic and molecular examination of the Drosophila zw10 gene. Mutants in the zw10 gene disrupt chromosome segregation in both mitotic and male meiotic cells. Much of the interest in the ZW10 protein stems from its dynamic localization during mitosis. The PI and his collaborators argue that this protein may play a critical role in mediating kinetochore function and in signaling, or responding to, the presence or absence of tension on the kinetochore. The zw10 gene encodes an 85 kd protein with no significant homologies to other proteins of known function. The PI proposes a clever set of experiments designed to elucidate the function of this protein. Specifically, he proposes to (1) use immuno-EM and RT videomicroscopy to follow the temporal and spatial distribute of ZW10 at very high resolution; (2) investigate the consequences of injecting anti-ZW10 antibodies at various times; (3) assay the effects on ZW10 distribution of altering tension on chromosomes by Iaser surgery and by micro-manipulation; (4) determine whether modifications of the ZW10 protein (e.g. phosphorylation) are associated with its movements during the cell cycle; (5) perform a structure/function dissection of ZW10; (6) study the function of the ZW10 protein in C elegans.; (7) investigate the relationship of ZW10 to the products of other known genes; (8) identify proteins that interact with ZW10 by now standard biochemical and genetic means; and (9) begin the molecular analysis of a new gene, san, whose protein product appears to play an essential role in sister chromatid cohesion.

该提案的重点是遗传和分子检查 果蝇 zw10 基因。 zw10 基因破坏的突变体 有丝分裂细胞和雄性减数分裂细胞中的染色体分离。 人们对 ZW10 蛋白的兴趣很大程度上源于其动态 有丝分裂期间的定位。 PI 和他的合作者争论 该蛋白质可能在介导着丝粒中发挥关键作用 功能以及发出信号或响应存在或 动粒上没有张力。 zw10 基因编码 85 kd 蛋白质，与其他蛋白质没有显着同源性 已知函数。 PI 提出了一组巧妙的实验 旨在阐明该蛋白质的功能。 具体来说，他 建议 (1) 使用免疫电子显微镜和 RT 视频显微镜 遵循ZW10在极高处的时间和空间分布 解决; (2)调查注射抗ZW10的后果 不同时间的抗体； (3) 测定对ZW10的影响 激光手术改变染色体张力的分布 并通过微操作； (4)判断是否修改 ZW10 蛋白的修饰（例如磷酸化）与其 细胞周期中的运动； (5) 执行结构/功能 ZW10的解剖； (6)研究ZW10蛋白的功能 秀丽隐杆线虫。 (7) 研究ZW10与 其他已知基因的产物； (8) 识别相互作用的蛋白质 通过现在标准的生化和遗传手段使用ZW10；和 (9) 开始对一个新基因 san 进行分子分析，该基因 蛋白质产品似乎在姐妹中起着至关重要的作用 染色单体凝聚力。