A prototype micro-fermentation platform for microbial strain and process development.

用于微生物菌株和工艺开发的原型微发酵平台。

• 批准号: 720075
• 金额: $ 31.86万
• 依托单位: TAP BIOSYSTEMS LIMITED
• 依托单位国家: 英国
• 项目类别: GRD Development of Prototype
• 财政年份: 2012
• 资助国家: 英国
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=720075
• 关键词: prototype; micro; fermentation; platform; microbial

A key need in the bioprocessing sector is for systems that can reduce the costs and timesassociated with the development of biological manufacturing processes. This would increasecompetitiveness through reduced times to market and improve quality and production cost andefficiency.This project aims to deliver a working prototype of an innovative bioprocessing platform thatmeets growing needs in diverse industry sectors. The instrument will run up to 48independent micro-bioreactors in parallel (10-15 ml each) for the development andoptimisation of industrial microbial processes. The functionality of this instrument aims toenable users to rapidly and cost effectively screen organisms (or strains) and growthparameters (e.g. media) and optimise the culture conditions before progressing to largeconventional fermentors. At present developers undertake such early processes in nonbioreactorconditions, such as shake flasks which lack control systems and are sub-optimalsince the conditions used do not properly reflect larger scale manufacturing conditions. Forthese reasons, current biomanufacturing processes and their development are often inefficientand sub-optimalEach small-scale bioreactor will be a single-use disposable item supplied in a sterile, ready-touseformat, removing the need for sterilisation and cleaning at instrument turnaround betweenexperiments. Current alternate strategies using numerous reusable bench-scale fermentors areslow due to cleaning, sterilisation and complex set-up activities and impose high labourburdens during set-up and operations.The system described will reduce the labour required to carry out such experimentation andincrease the number of experiments possible by an order of magnitude. Adopters of theinstrumentation platform developed in this project will improve their competitiveness byreducing the cost of development of improved processes and, critically, reducing thedevelopment cycle time.

生物加工领域的一个关键需求是能够降低与生物制造工艺开发相关的成本和时间的系统。这将通过缩短上市时间、提高质量、生产成本和效率来提高竞争力。该项目旨在提供一个创新生物加工平台的工作原型，以满足不同行业日益增长的需求。该仪器将并行运行多达48个独立的微型生物反应器（每个10-15 ml），用于工业微生物过程的开发和优化。该仪器的功能旨在使用户能够快速和经济有效地筛选生物体（或菌株）和生长参数（例如培养基），并在进入大型传统发酵罐之前优化培养条件。目前，开发人员在非生物反应器条件下进行这种早期过程，例如缺乏控制系统的摇瓶，并且是次优的，因为所使用的条件不能正确反映大规模生产条件。由于这些原因，目前的生物制造工艺及其发展往往是低效和次优的。每个小规模的生物反应器将是一个一次性使用的项目，以无菌，即用型的形式提供，消除了在实验之间的仪器周转时进行灭菌和清洁的需要。目前使用许多可重复使用的实验室规模发酵罐的替代策略由于清洁、灭菌和复杂的设置活动而很低，并且在设置和操作期间施加了很高的劳动力负担。所描述的系统将减少进行此类实验所需的劳动力，并将实验数量增加一个数量级。在这个项目中开发的仪器平台的制造商将通过降低改进过程的开发成本来提高他们的竞争力，关键是减少开发周期时间。