FUNCTION OF 95F UNCONVENTIONAL MYOSIN IN DROSOPHILA

这项提案中概述的研究使用果蝇来确定角色 与肌动蛋白合作的蛋白质介导肌动蛋白细胞骨架的 参与正常的细胞生理学。尤其是这个角色， 细胞骨架系统在动态过程中对 将检查有机体的发育情况。这项提案的重点是 是一种新的非传统肌球蛋白，称为果蝇95F 非常规肌球蛋白，存在于大多数(如果不是全部)细胞中 在苍蝇的整个生命周期中。有证据表明，它起到了 在胚胎早期发育中起重要作用。要检查 该蛋白在果蝇生活史中的功能研究 要使用此模型中提供的各种工具执行 有机体。首先，编码基因突变的影响 将对肌球蛋白重链进行分析，以确定何时和在哪个部位 组织中这种蛋白质起着重要作用。此外，生物化学特性还包括 该蛋白质将在体内通过体外功能测试进行检测。 可视化技术的过程中，这种蛋白质 参与，并使用抗体和其他 试剂。肌球蛋白家族的机械力化学酶包含不同的 一组蛋白质，被认为调节各种重要的 细胞动力学过程。然而，每一个人的特殊作用 目前尚不清楚这个家庭的许多不同成员。它很可能是 本申请中提出的实验将提供有关 肌球蛋白在多种组织和不同组织中的特殊作用 生物体发育的各个阶段；因此，这些实验将提供 对细胞如何参与生物体功能的重大洞察，如 以及提供关于特定的肌动蛋白过程的信息 它们的正常功能需要不同的细胞。

The studies outlined in this proposal use Drosophila to determine the role of proteins that cooperate with actin to mediate the actin cytoskeleton's participation in normal cell physiology. In particular, the role of this cytoskeletal system in dynamic processes that are important for development of the organism will be examined. The focus of this proposal is an novel unconventional myosin, called the Drosophila 95F unconventional myosin, that is present (in most if not all cells) throughout the life cycle of the fly. Evidence suggests that it plays an important role in early development of the embryo. To examine the function of this protein during the life cycle of Drosophila, the studies to be performed utilize a variety of tools available in this model organism. Primarily, the effects of mutation in the gene that encodes this myosin heavy chain will be analyzed to determine when and in which tissues this protein plays a role. In addition, biochemical properties of the protein will be examined, using in vitro assays for function, in vivo techniques for visualization of processes in which this protein participates, and disruption of function using antibodies and other reagents. The myosin family of mechanochemical enzymes contains a diverse group of proteins that are thought to mediate a variety of important cellular dynamic processes. However, the particular role of each of the many different members of the family remains unclear. It is likely the experiments proposed in this application will provide information on the particular role of this myosin in a variety of tissues and at different stages of organism development; thus, these experiments will provide significant insight into how cells participate in organism function, as well as to provide information on the particular actin-based process required in different cells for their normal function.