TERMINAL DIFFERENTIATION OF EPIDERMAL AND ADIPOSE CELLS

这个实验室致力于研究人类角质形成细胞；它的 增殖、末端分化及其特异基因 表情。角质形成细胞是终末期 分化细胞类型，对肿瘤性改变易感性高。 因为细胞类型现在非常有效地培养，并且表现出许多 分化特性在培养中，大多数工作使用细胞培养。 然而，这些培养物很容易移植到无菌动物身上 它们会产生正常的人类表皮。这是一项非常敏感的测试 正常角质形成细胞；病毒对培养细胞的转化 癌基因导致分化的肿瘤细胞发生非常明显的扰动 移植上皮的结构。没有其他组织可以 很容易从培养的细胞中产生，并通过这种方式进行研究。 大部分工作将集中在受调控的基因表达上，使用 总状蛋白和其他末端分化的特化蛋白质。在……里面 这项工作，研究了转基因基因及其在体内的表达 小(增殖)和大(终末分化)细胞， 通过淘洗分离出来的，已经表明基因是通过 组织和细胞隔室的特异性。治疗后基因表达的变化 将对培养细胞的移植进行研究。启动基因的信号 在大细胞中的表达必须与那些阻止 增殖，因为改变的基因表达是发展的基础 肿瘤。

This laboratory is devoted to the study of the human keratinocyte; its multiplication, its terminal differentiation, and its specific gene expression. The keratinocyte is a unique example of a terminally differentiating cell type with high susceptibility to neoplastic change. Because the cell type is now so efficiently cultivable and exhibits many differentiated properties in culture, most of the work uses cell cultures. However, the cultures are readily transplantable to athymic animals where they generate normal human epidermis. This is a very sensitive test for normal keratinocytes; transformation of the cultured cells with a viral oncogene results in very evident perturbation of the differentiated structure of the grafted epithelium. There is no other tissue which can be easily generated from cultured cells and studied this way. Much of the work will be focused on regulated gene expression, using involucrin and other specialized proteins of terminal differentiation. In this work, study of transfected genes and their resulting expression in small (proliferating) and large (terminally differentiating) cells, separated by elutriation, has already shown that genes are expressed with tissue and cell compartment specificity. Changes in gene expression after grafting of cultured cells will be studied. The signals that turn on gene expression in large cells must be closely related to those that arrest proliferation, since altered gene expression is basic to the development of neoplasia.