Studies on the Components and Bioactivities of Euphorbiacea plants wbich influencing Cell Cycle and Cell Differentiation

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The result in this research is as follows.1. Compound separated from kansui :On the basis of a bioassay-guided fractination, 21 sorts of ingenol type diterpenes which containing ten new compounds, five jatrophane type diterpenes which containing three new compounds, seven euphane/tirucallane type triterpenes which containing five new compounds, five steroid, one cumarin, one fatty acid, a total of 38 sorts of compounds were isolated and identified.2. Ingenol type diterpenes showed the strongest cell division inhibitory activity in the above-mentioned compounds. Moreover, these compounds controlled cell division and the cells survived.3. As a result of the selective inhibition of the growth of cancer cells (MMT, LC540, and 3T3) by ingenane-type diterpenes. 20-O-(2&cent ; E, 4&cent ; Z-decadienoyl) ingenol. (2) did not show a inhibition control to 3T3 cell which is a normal cell, but it showed the alternative inhibitory control action to the breast cancer cell MMT.4. Ingenol type diterpene 3-O-(2, 3-dimethylbutanoyl)-13-O-dodecanoylingenol (20) showed a cell growth inhibitory -activity remarkably to Jurkat and the HSC-2 cell. And the findings suggest 20 mainly induced early apoptosis in HSC-2 cells5. In the above results, ingenol type diterpene in kansui induced the early apotosis and inhibited cell division of some cancer-cells. It is thought that these bioactive components have a highly possibility to be an anti-cancer drug with a new action mechanism.