MEASUREMENT OF ALBUMIN SYNTHETIC RATE DURING RECOVERY FROM INTENSE EXERCISE

剧烈运动恢复期间白蛋白合成率的测量

• 批准号: 6247116
• 负责人: ETHAN R NADEL
• 金额: $ 2.68万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247116
• 关键词: albumins; analytical method; exercise; human subject; protein biosynthesis; stable isotope

We measured hepatic albumin synthesis during the recovery from an intense intermittent exercise protocol previously shown to expand plasma volume. Using a primed-constant infusion of a stable isotopic tracer of phenylalanine, hepatic fractional synthetic rate (FSR) and absolute synthetic rate (ASR) of albumin were determined from the enrichment of phenylalanine in albumin and in the free intravascular precursor pool. Albumin FSR and ASR were 6.39+0.48% day-1and 120 +9mg (kgBW)-1 day-1, respectively, at 3 to 6 hours recovery from exercise; the FSR and ASR on the time control study day were 5.94+0.47% day-1 and 104+9mg (kgBW) -1 day-1 respectively. The 6% and 16% increases (P<0.05) in FSR and ASR following exercise were associated with an elevated plasma albumin content at 5 and 6 hours recovery (P<0.05), an increased total protein content throughout recovery (P<0.05), and a negative free water clearance (P<0.05) at 2,3 and 6.5 hours of recovery when compared to baseline values; these variables were unchanged from their baselines on the time control study day. Increased albumin content and reduced free water clearance both contribute to a retention of fluid within the circulation after intense exercise. The measured increase in albumin synthesis did not account for the entire increase in albumin content during recovery from exercise. Although the measured increase in albumin synthesis did not account for the elevated plasma albumin content in the 6 hours following exercise, we concluded that it was elevated sufficiently to account for the increased albumin content measured (in a prior study) after 24 hours of recovery.

我们测量了在从高强度应激恢复过程中肝脏白蛋白的合成。 间歇性运动方案先前显示扩大血浆容量。 使用一种稳定的同位素示踪剂， 苯丙氨酸、肝脏合成率（FSR）和绝对值 白蛋白的合成速率（ASR）从富集的 白蛋白和游离血管内前体池中的苯丙氨酸。 白蛋白FSR和ASR分别为6.39 ± 0.48%和120 ± 9 mg（kgBW）-1天-1， 分别在运动后3至6小时恢复; FSR和ASR 时间对照研究天数分别为5.94 ± 0.47%天-1和104 ± 9 mg（kgBW）-1 第1天分别。 FSR和ASR分别增加6%和16%（P<0.05 运动后与血浆白蛋白含量升高有关 恢复后5、6 h，总蛋白含量显著增加（P<0.05）， 在整个恢复期（P<0.05），和负的自由水清除率（P<0.05） 与基线值相比，在恢复2、3和6.5小时时;这些 在时间控制研究中，变量与基线相比没有变化 天 白蛋白含量增加和游离水清除率降低， 有助于在强烈的血液循环后保留液体 锻炼的 测得的白蛋白合成增加并不能解释 运动恢复期间白蛋白含量的整体增加。 尽管测得的白蛋白合成增加并不能解释 运动后6小时血浆白蛋白含量升高， 结论是，它的升高足以解释增加的 恢复24小时后测量的白蛋白含量（在先前研究中）。