TNF ALPHA AND OTHER MEDIATORS IN THE PATHOGENESIS & PREDICTION OF PREECLAMPSIA

TNF α 和发病机制中的其他介质

• 批准号: 6246901
• 负责人: Men-Jean Lee
• 金额: $ 2.39万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-12 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6246901
• 关键词: clinical research; disease /disorder proneness /risk; endothelin; fibronectins; growth factor receptors; human data; human tissue; in situ hybridization; pathologic process; platelet activating factor; preeclampsia; tissue /cell culture; tumor necrosis factor alpha

The primary objective of this study is to investigate the role of cytokines, autocoids and other vascular derived substances in the pathogenesis of preeclampsia. TNF-alpha, fibronectin, endothelin and platelet activating factor (PAF) will be evaluated longitudinally in plasma, sera and amniotic fluid for their possible application in detecting patients at risk for preeclampsia. Levels of protein and gene expression via in situ hybridization and immunodetection for TNF-alpha,TNF receptor, fibronectin, fibronectin receptor, endothelin and PAF will be evaluated in placentas, uterine biopsies and umbilical cord derived endothelial cell monolayers. Blood from pregnant patients who are diagnosed with preeclampsia will be obtained from specimens originally collected for SPID # 733. Use of the GCRC designed data base and statistical analysis will be done as mentioned in SPID #733. The Core laboratory will be utilized for blood separation and laminar flow hoods.

本研究的主要目的是探讨 细胞因子、autocoids和其他血管衍生物质 先兆子痫的发病机制。 TNF-α、纤连蛋白、内皮素和 血小板活化因子（PAF）将在 血浆、血清和羊水的可能应用， 检测有先兆子痫风险的患者。蛋白质和基因水平 通过原位杂交和免疫检测TNF-α、TNF 受体、纤连蛋白、纤连蛋白受体、内皮素和血小板激活因子将 在胎盘、子宫活检和脐带来源的 内皮细胞单层。 孕妇的血液 诊断为先兆子痫的患者将从最初的标本中获得 SPID#733的收集。使用全球气候变化研究中心设计的数据库， 将按照SPID #733中所述进行统计分析。 核心 实验室将用于血液分离和层流罩。