TNF ALPHA AND OTHER MEDIATORS IN THE PATHOGENESIS & PREDICTION OF PREECLAMPSIA

TNF α 和发病机制中的其他介质

• 批准号: 6305938
• 负责人: Men-Jean Lee
• 金额: $ 2.1万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6305938
• 关键词: clinical research; disease /disorder proneness /risk; endothelin; fibronectins; growth factor receptors; human data; human tissue; in situ hybridization; pathologic process; platelet activating factor; preeclampsia; tissue /cell culture; tumor necrosis factor alpha

The primary objective of this study is to investigate the role of cytokines, autocoids and other vascular derived substances in the pathogenesis of preeclampsia. TNF-alpha, fibronectin, endothelin and platelet activating factor (PAF) will be evaluated longitudinally in plasma, sera and amniotic fluid for their possible application in detecting patients at risk for preeclampsia. Levels of protein and gene expression via in situ hybridization and immunodetection for TNF-alpha, TNF receptor, fibronectin, fibronectin receptor, endothelin and PAF will be evaluated in placentas, uterine biopsies and umbilical cord derived endothelial cell monolayers. Blood from pregnant patients who are diagnosed with preeclampsia will be obtained from specimens originally collected for SPID # 733. Use of the GCRC designed data base and statistical analysis will be done as mentioned in SPID #733.

本研究的主要目的是探讨细胞因子，autocoids和其他血管源性物质在先兆子痫发病机制中的作用。 将在血浆、血清和羊水中纵向评价TNF-α、纤连蛋白、内皮素和血小板活化因子（PAF），以探讨其在检测先兆子痫风险患者中的可能应用。将在胎盘、子宫活检和脐带来源的内皮细胞单层中通过原位杂交和免疫检测评价TNF-α、TNF受体、纤连蛋白、纤连蛋白受体、内皮素和PAF的蛋白和基因表达水平。 诊断为先兆子痫的妊娠患者的血液将从最初为SPID # 733采集的标本中获得。将按照SPID #733中所述使用GCRC设计的数据库和进行统计分析。