CARTILAGE MARKERS IN JOINT FLUID AND SERUM AFTER MENISCUS INJURY AND TREATMENT

半月板损伤和治疗后关节液和血清中的软骨标记

• 批准号: 6247023
• 负责人: Bruce Beynnon
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-23 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247023
• 关键词: articular cartilage; biomarker; cartilage metabolism; clinical research; early diagnosis; human subject; knee; magnetic resonance imaging; musculoskeletal imaging /visualization /scanning; musculoskeletal injury; osteoarthritis

Specific Aims; STUDY 1:(1)To compare the concentration of articular cartilage markers (aggrecan, stromelysin, tissue inhibitor of metalloproteinases (TIMP), procollagen II C-peptide, cartliage and oligiometric matrix protein (COMP) in joint fluid aggrecan, COMP, and keratan sulfate in serum) obtained from joint fluid of both knees with a posterior horn meniscus tear with the contralateral uninjured knee at arthroscopy. (2) To study the relation between articular cartliage marker concentrations and the cartilage condition observed with arthroscopy and MRI. (3) To determine the relationship between the patients persisting symptoms and function and the concentration of cartilage markers in joint fluid obtained from knees with a symptomatic meniscus tear, before and after 6 mon. rehab.(4) To determine the relationship between the patients persisting sympoms and function and the concentration of cartilage markers in joint fluid obtained from the contralateral normal knee at the same time intervals as used for the injured knee. STUDY 2:(1) To compare the concentrations of articular cartilage markers in joint fluid obtained from the right knee with concentrations of these markers in joint fluid from the left knee in subjects with normal MR images of their knees. (2) To compare the concentrations of articular cartilage markers in joint fluid obtained from the right and left knees in subjects with normal MR images of their knees with the concentrations of these markers in joint fluid from the injured knee and contralateral normal knee in patients with a meniscus tear. Joint fluid samples are brought to the GCRC Laboratory and are clarified by centrifugation. Aliquots are prepared and frozen in a GCRC freezer for later analysis.

具体目的：研究1：（1）比较关节炎的浓度， 软骨标记物（聚集蛋白聚糖、基质溶解素、软骨细胞生长因子的组织抑制剂）、 金属蛋白酶（TIMP）、前胶原II C-肽、软骨和 关节液聚集蛋白聚糖中的寡聚基质蛋白（COMP）， 血清中硫酸角质素）， 后角半月板撕裂，对侧未受伤膝关节， 关节镜检查(2)探讨关节软骨与关节功能的关系 标记物浓度和观察到的软骨状况， 关节镜检查和MRI。(3)为了确定 患者持续的症状和功能以及 从有症状的膝关节中获得的关节液中的软骨标记物 半月板撕裂，6个月前后。戒毒所(4)确定 患者持续症状与功能之间的关系， 软骨标记物在关节液中的浓度， 对侧正常膝关节在相同的时间间隔，用于 膝盖受伤研究2：（1）比较关节炎组和对照组的关节炎浓度， 从右膝获得的关节液中的软骨标记物， 这些标记物在左膝关节液中的浓度， 受试者的膝关节MR图像正常。(2)比较 获得的关节液中关节软骨标记物的浓度 从具有正常MR图像的受试者的右膝和左膝， 膝关节的关节液中这些标记物的浓度 半月板患者的损伤膝和对侧正常膝 眼泪。 将关节液样本带到GCRC实验室并进行澄清 通过离心分离。 制备等分试样，并在GCRC冰柜中冷冻 以便以后分析。