ANALYSIS OF IN VIVO HPRT MUTATIONS IN PREMATURE INFANTS

早产儿体内 HPRT 突变分析

• 批准号: 6247014
• 负责人: ROBERT A SILVER
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-23 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6247014
• 关键词: DNA; DNA damage; T lymphocyte; clinical research; gene frequency; gene mutation; gestational age; human genetic material tag; human subject; medical records; molecular cloning; mutant; neoplasm /cancer genetics; premature infant human; questionnaires

In this study, the T-cell cloning assay will be used to isolate hprt mutants in preterm infants. Mutant frequencies will be calculated in preterm infants of varying gestational ages (24-36 weeks). Through DNA analysis specific types of hprt mutations will be found. A questionnaire of social history and review of the medical history of the mother will be taken as well, which will be related to changes in mutant frequency in preterm infants. This will be compared to mutant frequencies known for full term infants and children. Data are being analyzed using the CDMAS resource.

在本研究中，T细胞克隆试验将用于分离hprt 早产儿中的突变体 突变频率将在 不同胎龄的早产儿（24-36周）。 通过DNA 分析特定类型的hprt突变将被发现。 社会史问卷和病史回顾 母亲也会被带走，这将与突变体的变化有关。 早产儿的频率。 这将与突变体进行比较 已知的足月婴儿和儿童的频率。 目前正在使用CDMAS资源分析数据。