HOX GENE INFLUENCE ON LATERAL PATTERNING IN VERTEBRATES

描述(改编自调查员摘要)：所解决的问题 是HOX基因在吻-尾轴中表达的作用 侧器官的基因表达以及这是否解释了该位置 侧器官的位置。有三个具体目标。首先，它是 建议确定是否将轴向HOX代码用于位置 胚胎外侧区域的信息。 第二个目标是绘制形成肩胛骨的个体体节的命运图。 根据早期的工作，首席调查员提出，皮肌刀 而不是长出肩胛骨的软骨细胞。 第三个目标将使用Hoxa-6、Hoxb-6和Hoxc-6的定向错误表达 进入节板，形成体节7-14。全长 将分离鸡Hoxa-6和Hoxb-6的克隆，创建RCAS载体。 基本上，这种方法将挑战系统，使其能够洞察 边界建立在胚胎的颈部区域。

DESCRIPTION (adapted from investigator's abstract): The question addressed is the role of Hox genes expressed in the rostro-caudal axis, in the Hox gene expression of lateral organs and whether this accounts for the position of the lateral organs. There are three specific aims. First, it is proposed to determine if the axial Hox code is used for positional information in the lateral regions of the embryo. The second aim is to fate map the individual somites that form the scapula. From earlier work the Principal Investigator proposes that the dermamyotome rather than the sleratome gives rise to the chondrocytes of the scapula. The third aim will use targeted misexpression of Hoxa-6, Hoxb-6 and Hoxc-6 into the segmental plate that will give rise to somites 7-14. Full length clones of chick Hoxa-6 and Hoxb-6 will be isolated, RCAS vectors created. Basically this approach will challenge the system to permit insight into how boundaries are established in the cervical region of the embryo.