HOX GENE INFLUENCE ON LATERAL PATTERNING IN VERTEBRATES

描述（改编自研究者摘要）：解决的问题 是在喙尾轴中表达的Hox基因的作用， 侧器官的基因表达以及这是否解释了 侧部器官。 有三个具体目标。 一是 建议确定轴向Hox代码是否用于位置 在胚胎的侧面区域的信息。 第二个目标是命运地图的个人体节，形成肩胛骨。 根据早期的工作，主要研究者提出， 而不是骨刀产生肩胛骨的软骨细胞。 第三个目标将使用Hoxa-6、Hoxb-6和Hoxc-6的靶向错误表达 进入节板，这将产生体节7-14。 全长 将分离鸡Hoxa-6和Hoxb-6的克隆，产生RCAS载体。 基本上，这种方法将挑战系统，以允许洞察如何 在胚胎的子宫颈区域建立边界。

DESCRIPTION (adapted from investigator's abstract): The question addressed is the role of Hox genes expressed in the rostro-caudal axis, in the Hox gene expression of lateral organs and whether this accounts for the position of the lateral organs. There are three specific aims. First, it is proposed to determine if the axial Hox code is used for positional information in the lateral regions of the embryo. The second aim is to fate map the individual somites that form the scapula. From earlier work the Principal Investigator proposes that the dermamyotome rather than the sleratome gives rise to the chondrocytes of the scapula. The third aim will use targeted misexpression of Hoxa-6, Hoxb-6 and Hoxc-6 into the segmental plate that will give rise to somites 7-14. Full length clones of chick Hoxa-6 and Hoxb-6 will be isolated, RCAS vectors created. Basically this approach will challenge the system to permit insight into how boundaries are established in the cervical region of the embryo.