CONTROL OF MICROTUBULE DYNAMICS DURING CELL CYCLE

细胞周期中微管动力学的控制

• 批准号: 2429873
• 负责人: BIN-BING ZHOU
• 金额: $ 2.99万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2429873
• 关键词: Xenopus oocyte; cell cycle; cell growth regulation; microtubules; mitotic spindle apparatus; video microscopy

Microtubule dynamics change dramatically during the cell cycle, from the relatively stable interphase microtubules to the very dynamic mitotic spindle. Studies with cell-free extracts of Xenopus eggs have shown that the interphase-mitosis transition of microtubule dynamics involves regulation of catastrophe rate, which is induced by phosphorylation reactions mediated by cdc2 kinase. However, both catastrophe factors and substrate proteins for these kinases that may play key roles in the change of microtubule dynamics remain to be identified. To quantitate the catastrophe activities and purify the potential catastrophe factors, I have developed a simple pulsed-chase assay to measure the catastrophe rate. Using this assay, I have shown that the catastrophe factors are active in both mitotic and interphase extracts, but the activities seem modulated during the cell cycle. The objective of this proposal is to purify the catastrophe factors, study the roles of such factors in the regulation of microtuble dynamics and spindle morphogenesis. To characterize the microtubule dynamics, both biochemical assay and real time video microscopy will be used. Since microtubule plays vital role in the cell cycle regulation, therefore regulation of proliferation, this project is directly related to cancer research.

微管动力学在细胞周期中发生显著变化， 相对稳定的间期微管， 纺锤体。对非洲爪蟾卵的无细胞提取物的研究表明， 微管动力学的间期-有丝分裂转变包括 突变率的调节，这是由磷酸化诱导的 由CDC 2激酶介导的反应。然而，灾难因素和 这些激酶的底物蛋白可能在这种变化中起关键作用， 微管动力学的研究仍有待进一步研究。以定量 灾害活动，净化潜在的灾害因素， 开发了一种简单的脉冲追踪试验来测量 率使用这种分析，我已经证明了突变因子是 在有丝分裂和间期提取物中都有活性，但这些活性似乎 在细胞周期中被调节。这项建议的目的是 提纯突变因子，研究突变因子在 微管动力学和纺锤体形态发生的调节。到 表征微管动力学，包括生化测定和真实的 将使用时间视频显微镜。由于微管在 细胞周期调节，因此调节增殖， 该项目与癌症研究直接相关。