TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
批准号:
2382821
负责人:
MICHAEL J MCMANUS
金额:
$9.22万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-08-20 至 2002-07-31
关键词:
affinity chromatography biological signal transduction caldesmon carcinogenesis chick embryo fibroblasts laboratory mouse laboratory rabbit molecular cloning oncogenes oncoproteins phosphorylation protein purification protein structure function protein tyrosine kinase recombinant virus site directed mutagenesis transfection virus genetics western blottings
中文摘要
禽红母细胞病病毒的v-erbB癌基因
英文摘要
DESCRIPTION: The v-erbB oncogene of the avian erythroblastosis virus
encodes a truncated growth factor receptor with tyrosine kinase activity.
Expression of this viral oncogene causes fibrosarcomas, erythroleukemias and
hemangiosarcomas. Understanding the mechanisms of signal transduction by
the v-ErbB oncoprotein will offer insights into tyrosine kinase signal
transduction pathways, into the formation of transformation-specific
multiprotein complexes, and has great potential to offer insights into the
molecular biology of human malignancies. With these long-term goals in
mind, the experiments outlined in this proposal will use well-characterized,
transformation-specific, structural mutants of v-ErbB to dissect tyrosine
kinase signaling pathways in transformed fibroblasts. This approach has
already revealed the formation of a transformation-specific multiprotein
signaling complex in v-ErbB transformed fibroblasts. This complex is
composed of two signal adapter molecules (SHC and GRB2), a guanine
nucleotide exchange factor (SOS), a tyrosine phosphorylated form of the
cytoskeletal regulatory protein caldesmon, and a novel tyrosine
phosphorylated protein (pp75). The hypothesis to be tested is that the
oncogenic signals initiated by v-ErbB are transduced through the
SHC-GRB2-SOS-caldesmon-pp75 complex, and that tyrosine phosphorylated
caldesmon and pp75 participate in the regulation of anchorage-independent
growth and tumorigenicity. The specific aims of this study are to (I)
determine the mechanism of association of caldesmon with the multiprotein
complex, (ii) define the functional role of caldesmon in v-ErbB mediated
transformation, and (iii) characterize the novel 75 kd tyrosine
phosphorylated protein in this signaling complex.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Very High Dimensional Visual Mining of the NCI Dataset
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批准号:6443134
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项目类别:
-
资助金额:$9.92万
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财政年份:2002
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
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批准号:6376494
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项目类别:
-
资助金额:$13.37万
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财政年份:1997
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
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批准号:2748954
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项目类别:
-
资助金额:$9.59万
-
财政年份:1997
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
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批准号:2896118
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项目类别:
-
资助金额:$9.98万
-
财政年份:1997
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TRANSFORMATION SPECIFIC SIGNALING MEDIATED BY VERBS
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批准号:6345476
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项目类别:
-
资助金额:$12.86万
-
财政年份:1997
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
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批准号:3034565
-
项目类别:
-
资助金额:$0.69万
-
财政年份:1992
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负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
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批准号:2084184
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项目类别:
-
资助金额:$6.67万
-
财政年份:1992
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
-
批准号:2084183
-
项目类别:
-
资助金额:$6.11万
-
财政年份:1992
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
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批准号:2084185
-
项目类别:
-
资助金额:$7.17万
-
财政年份:1992
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
-
批准号:3085948
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项目类别:
-
资助金额:$6.61万
-
财政年份:1992
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
-
批准号:3085949
-
项目类别:
-
资助金额:$4.97万
-
财政年份:1992
-
负责人:MICHAEL J MCMANUS
-
依托单位:
TISSUE-SPECIFIC TUMORIGENESIS BY THE C-ERBB ONCOGENE
-
批准号:3034564
-
项目类别:
-
资助金额:$3.53万
-
财政年份:1991
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负责人:MICHAEL J MCMANUS
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依托单位:
海外基金