MOLECULAR MECHANISMS OF ESTROGEN INDUCED CARCINOGENESIS

说明(改编自申请人的摘要)： 此应用程序的建议假设假设了三个关键步骤 在叙利亚仓鼠肾脏肿瘤模型中的致癌研究：1) 激素刺激，2)代谢激活，3)自由基产生。 本研究的具体目的是：1)克隆和鉴定叙利亚仓鼠ER基因； 2)研究内质网代谢活化/自由基生成的作用 基因表达；3)表征代谢/雌激素潜能的作用 ER蛋白在仓鼠肿瘤发生中的细胞特异性表达 肾脏；以及4)研究雌激素代谢激活的作用 以及过度表达和neu之间的相互依存关系(如果有的话) 压制。

DESCRIPTION (Adapted from the applicant's abstract): The foundation for the proposed hypothesis of this application assumes three critical steps to carcinogenesis as studied in the Syrian hamster kidney tumor model: 1) hormonal stimulation, 2) metabolic activation, 3) free radical generation. The specific aims are to: 1) clone and characterize Syrian hamster ER cDNA; 2) study the effect of metabolic activation/free radical generation of ER gene expression; 3) characterize the role of metabolic/estrogenic potential on cell specific expression of ER proteins on tumorigenesis in hamster kidneys; and 4) investigate the role of metabolic activation of estrogens and the interdependency of, if any, between overexpression and neu suppression.