PHOTOCLEAVABLE REAGENTS FOR ISOLATION OF STEM CELLS

干细胞的分离是用于治疗 各种各样的疾病。 由于干细胞可以增殖， 分化，它们的分离有利于移植和基因 治疗以及了解造血的基本过程。 虽然通过体外培养分离干细胞已经取得了进展， 基于生物素-亲和素的免疫吸附和荧光激活细胞 分选，仍然需要改进以提高产率、纯度 和快速处理干细胞。 AmberGen的最新进展， Inc.在光可裂解试剂领域中， 提议的研究。 可光裂解的生物素允许特定的细胞 通过生物素-亲和素从异质群体中分离 相互作用，然后通过光释放。 光可裂解标记物可以是 通过抗体附着到细胞上进行细胞分选，然后通过 光 最近的进展表明，这些光试剂可以被设计成 在对细胞无损伤的红移波长下光裂解。 研究还将针对小说的设计和制作 一类抗体，其与抗原的相互作用可通过 光 AmberGen公司将综合和评估各种 光可裂解生物素、光可裂解标记物和光活化氨基 用于分离干细胞的酸。 拟议的商业应用：开发基于 光可切割生物素（PC-Biotin TM）、光可切割标记物（PC-Marker TM）和光调制抗体（LM-抗体TM）将具有商业上的 用于分离和检测多种细胞的应用 包括干细胞和病原体。 潜在产品包括PC-生物素 TM和PC-Marker TM化合物，antibody-PC-Biotin TM/antibody-PC-Marker TM 缀合物、光可裂解的FACS标记、用于细胞分离的试剂盒和 用于细胞分离的自动化设备。

The isolation of stem cells is a critical technology for the treatment of a wide range of diseases. Since stem cells can proliferate and differentiate, their isolation facilitates transplantation and gene therapies as well as understanding the basic process of hematopoiesis. While progress has been made in the isolation of stem cells through biotin-avidin based immunoadsorption and fluorescence-activated cell sorting, improvements are still needed in order to increase yields, purity and rapidity of processing of stem cells. Recent advances by AmberGen, Inc. in the field of photocleavable reagents provide the basis of the proposed research. Photocleavable biotins allow specific cells to be separated from a heterogeneous population through the biotin-avidin interaction and then released by light. Photocleavable markers can be attached to cells through antibodies for cell sorting and then removed by light. Recent progress shows these photoreagents can be designed to photocleave at red-shifted wavelengths which are non-damaging to cells. Research will also be directed at the design and production of a novel class of antibodies whose interaction with antigens can be modulated by light. AmberGen, Inc. will synthesize and evaluate a variety of photocleavable biotins, photocleavable markers and light-activated amino acids for the isolation of stem cells. PROPOSED COMMERCIAL APPLICATION: Development of new technology based on photocleavable biotins (PC-Biotin TM), photocleavable markers (PC-Marker TM) and Light modulated antibodies (LM-Antibodies TM) will have commercial applications for the isolation and detection of a wide variety of cells including stem cells and pathogens. Potential products include PC-Biotin TM and PC-Marker TM compounds, antibody-PC-Biotin TM/antibody-PC-Marker TM conjugates, photocleavable FACS labels, kits for cell isolation and automated devices for cell separation.