PHOTOCLEAVABLE REAGENTS FOR ISOLATION OF STEM CELLS

用于分离干细胞的光裂解试剂

• 批准号: 2005309
• 负责人: JERZY OLEJNIK
• 金额: $ 10万
• 依托单位: AMBERGEN, INC
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2005309
• 关键词: antibody; binding proteins; biomarker; biotin; cell sorting; chemical cleavage; chemical synthesis; embryonic stem cell; flow cytometry; photochemistry; reagent /indicator

The isolation of stem cells is a critical technology for the treatment of a wide range of diseases. Since stem cells can proliferate and differentiate, their isolation facilitates transplantation and gene therapies as well as understanding the basic process of hematopoiesis. While progress has been made in the isolation of stem cells through biotin-avidin based immunoadsorption and fluorescence-activated cell sorting, improvements are still needed in order to increase yields, purity and rapidity of processing of stem cells. Recent advances by AmberGen, Inc. in the field of photocleavable reagents provide the basis of the proposed research. Photocleavable biotins allow specific cells to be separated from a heterogeneous population through the biotin-avidin interaction and then released by light. Photocleavable markers can be attached to cells through antibodies for cell sorting and then removed by light. Recent progress shows these photoreagents can be designed to photocleave at red-shifted wavelengths which are non-damaging to cells. Research will also be directed at the design and production of a novel class of antibodies whose interaction with antigens can be modulated by light. AmberGen, Inc. will synthesize and evaluate a variety of photocleavable biotins, photocleavable markers and light-activated amino acids for the isolation of stem cells. PROPOSED COMMERCIAL APPLICATION: Development of new technology based on photocleavable biotins (PC-Biotin TM), photocleavable markers (PC-Marker TM) and Light modulated antibodies (LM-Antibodies TM) will have commercial applications for the isolation and detection of a wide variety of cells including stem cells and pathogens. Potential products include PC-Biotin TM and PC-Marker TM compounds, antibody-PC-Biotin TM/antibody-PC-Marker TM conjugates, photocleavable FACS labels, kits for cell isolation and automated devices for cell separation.

干细胞的分离是治疗糖尿病的关键技术

项目成果

The cross-clade neutralizing activity of a human monoclonal antibody is determined by the GPGR V3 motif of HIV type 1.

人单克隆抗体的跨进化枝中和活性由 1 型 HIV 的 GPGR V3 基序决定。

• DOI: 10.1089/aid.2004.20.1254
• 发表时间: 2004
• 期刊: AIDS research and human retroviruses.
• 作者: Zolla-Pazner,Susan;Zhong,Ping;Revesz,Kathy;Volsky,Barbara;Williams,Constance;Nyambi,Phillipe;Gorny,MiroslawK
• 通讯作者: Gorny,MiroslawK