PHOTOCLEAVABLE REAGENTS FOR ISOLATION OF STEM CELLS

干细胞的分离是治疗癌症的一项关键技术。 各种各样的疾病。因为干细胞可以增殖和 分化，它们的分离促进了移植和基因 治疗以及了解造血的基本过程。 虽然在干细胞分离方面取得了进展，但通过 基于生物素-亲和素的免疫吸附和荧光激活细胞 分选，仍然需要改进，以提高产量、纯度 和干细胞处理的速度。AmberGen的最新进展， Inc.在光解试剂领域提供了基础 拟开展的研究。可光切割的生物素允许特定细胞 通过生物素亲和素从异质种群中分离出来 相互作用，然后被光释放。可光切割的标记可以是 通过抗体附着在细胞上进行细胞分选，然后通过 灯。最新进展表明，这些光试剂可以设计成 光分裂在红移的波长，这对细胞是无害的。 研究还将针对一部小说的设计和制作 一类抗体，其与抗原的相互作用可由 灯。AmberGen，Inc.将综合和评估各种 光解生物素、光解标记和光活化氨基 用于分离干细胞的酸。 建议的商业应用：基于 光解生物素(PC-Biotin TM)、光解标记(PC-Marker TM)和光调制抗体(LM-抗体TM)将有商业化 在分离和检测多种细胞方面的应用 包括干细胞和病原体。潜在产品包括PC-Biotin TM和PC-Marker TM化合物，抗体-PC-生物素TM/抗体-PC-Marker TM 结合物、可光裂解的流式细胞仪标记、细胞分离试剂盒和 用于细胞分离的自动化设备。

The isolation of stem cells is a critical technology for the treatment of a wide range of diseases. Since stem cells can proliferate and differentiate, their isolation facilitates transplantation and gene therapies as well as understanding the basic process of hematopoiesis. While progress has been made in the isolation of stem cells through biotin-avidin based immunoadsorption and fluorescence-activated cell sorting, improvements are still needed in order to increase yields, purity and rapidity of processing of stem cells. Recent advances by AmberGen, Inc. in the field of photocleavable reagents provide the basis of the proposed research. Photocleavable biotins allow specific cells to be separated from a heterogeneous population through the biotin-avidin interaction and then released by light. Photocleavable markers can be attached to cells through antibodies for cell sorting and then removed by light. Recent progress shows these photoreagents can be designed to photocleave at red-shifted wavelengths which are non-damaging to cells. Research will also be directed at the design and production of a novel class of antibodies whose interaction with antigens can be modulated by light. AmberGen, Inc. will synthesize and evaluate a variety of photocleavable biotins, photocleavable markers and light-activated amino acids for the isolation of stem cells. PROPOSED COMMERCIAL APPLICATION: Development of new technology based on photocleavable biotins (PC-Biotin TM), photocleavable markers (PC-Marker TM) and Light modulated antibodies (LM-Antibodies TM) will have commercial applications for the isolation and detection of a wide variety of cells including stem cells and pathogens. Potential products include PC-Biotin TM and PC-Marker TM compounds, antibody-PC-Biotin TM/antibody-PC-Marker TM conjugates, photocleavable FACS labels, kits for cell isolation and automated devices for cell separation.