Proteome Array for Global Mapping of Cellular Pathways

描述（由申请人提供）：基于人类基因组测序的最新进展，出现了一个新的目标：表达和分析人类蛋白质组。蛋白质组阵列的发展将加速生物学和医学几个关键领域的进展，包括绘制细胞通路，发现新的药物靶点和识别疾病相关的生物标志物。然而，蛋白质组阵列的生产目前受到来自基因文库表达蛋白质、用于位点特异性表面附着的工程蛋白质和检测多种蛋白质-蛋白质相互作用的改进方法的阻碍。该项目旨在通过结合无细胞方法快速表达特殊工程蛋白库和先进的微阵列制造和读出技术来克服这些限制。AmberGen将生产和评估由无细胞表达蛋白文库构建的蛋白质组阵列，设计用于高灵敏度和多重读出。蛋白质-蛋白质相互作用的快速检测将用于绘制细胞通路和发现新的生物标志物。从病原体幽门螺杆菌和从人类基因组中选择的基因中构建和评估原型蛋白质组阵列，将在第一阶段进行。第二阶段将侧重于自身免疫性疾病和传染病领域的应用。

DESCRIPTION (provided by applicant): Based on recent progress in sequencing the human genome, a new goal has emerged: to express and analyze the human proteome. The development of proteome arrays will accelerate progress in several key areas of biology and medicine including mapping cellular pathways, discovering new drug targets and identifying disease related biomarkers. However, the production of proteome arrays is currently hindered by the need for improved methods of expressing proteins from gene libraries, engineering proteins for site-specific surface attachment and detecting multiple protein-protein interactions. This project aims to overcome these limitations by combining cell-free methods for rapid expression of specially engineered protein libraries with advanced technology for microarray fabrication and readout.AmberGen will produce and evaluate proteome arrays constructed from cell-free expressed protein libraries and designed for high sensitivity and multiplex readout. The rapid detection of protein-protein interactions will be used to map cellular pathways and to discover new biomarkers. Prototype proteome arrays from the pathogen H. pylori and selected genes from the human genome will be constructed and evaluated in Phase I. Phase II will focus on applications in the areas of autoimmune and infectious diseases.