RAPID IDENTIFICATION OF FUNGAL SPECIES

真菌种类的快速鉴定

• 批准号: 2422343
• 负责人: MEAD M MCCABE
• 金额: $ 9.99万
• 依托单位: GENETIC VECTORS, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2422343
• 关键词: Candida; Cryptococcus; Saccharomyces cerevisiae; enzyme linked immunosorbent assay; method development; microorganism classification; nucleic acid sequence; polymerase chain reaction; yeasts

Amplification primers specific for yeast species and a rapid microplate assay for detection of PCR product will be developed in these studies. The B region of the large ribosomal subunit DNA (LrDNA) of 24 yeast species will be sequenced and computer alignment against known sequences will be used to select species-specific regions of LrDNA for use as PCR primer oligonucleotides. A 3-primer PCR technique (2 universal primers and one species primer) will be used to validate the usefulness of the primer for species identification. Concurrently, a microplate assay for detection of PCR product will be developed and tested using the panel of primers. The PCR primers and the microplate assay will be combined to produce assay kits for detection of fungal species. The target markets include clinical and veterinary diagnostics, industrial quality control and basic research. PROPOSED COMMERCIAL APPLICATION: Diagnostic kits for fungal identification will be developed. Target markets include clinical, veterinarian, industrial quality control and basic and applied research.

酵母菌种特异性扩增引物和快速微孔板 在这些研究中将开发用于检测PCR产物的测定法。 24酵母核糖体大亚基DNA（LrDNA）的B区 物种将被测序，并与已知序列进行计算机比对。 将用于选择LrDNA的物种特异性区域，以用作PCR 引物寡核苷酸。 3引物PCR技术（2个通用引物 和一个物种引物）将用于验证 种鉴定引物。 与此同时，进行了微量板测定， PCR产物的检测将使用 引物 将PCR引物和微孔板试验组合， 生产检测真菌种类的检测试剂盒。 目标市场 包括临床和兽医诊断、工业质量控制 和基础研究。 拟议的商业应用：真菌诊断试剂盒 将开发识别。 目标市场包括临床， 兽医、工业质量控制以及基础和应用研究。