设计与构建胞内油脂联产胞外附加值化学品体系对于提升油脂合成体系的原子收率和经济值具有重要意义，然而目前缺乏对于联产体系内碳代谢通量的高效配置研究。申请人在前期研究中筛选获得一株可以联产胞内油脂与胞外葡萄糖酸的隐球酵母Cryptococcus podzolicus DSM27192。本项目拟以该菌株为研究对象，通过对其代谢组学与转录组学研究，解析联产体系内的碳代谢流分配机制；在此基础上，通过代谢工程改造，匹配与平衡油脂与葡萄糖酸合成途径中的辅因子系统，提高联产体系的产品收率；进一步通过优化碳代谢通量在油脂与葡萄糖酸合成途径的分布，提高联产体系的生产强度，最终实现联产体系产品的高效合成。通过对C. podzolicus DSM27192联产体系碳代谢流的分配机制解析与调控研究，为设计与构建油脂联产其他胞外附加值化学品体系提供理论指导和技术支持，为微生物油脂的产业化提供新的思路。

Designing and constructing a co-generation system of lipid (intracellular) and value-added chemicals (extracellular) shows great significance in improving the economics of microbial lipid production process. However, there is a lack of efficient carbon metabolic flux allocation in the studies of lipid co-generation systems. In this project, an oleaginous yeast Cryptococcus podzolicus DSM27192 with the capability of co-generating microbial lipid and gluconic acid was firstly isolated and characterized. A comprehensive investigation of its metabolomics and transcriptomics will be carried out to analyze the distribution mechanism of carbon flux in this co-generation system. Further, the co-factor system related to lipid synthesis pathway and gluconic acid synthesis pathway will be reformed and balanced to improve the total products yield in this co- generation system. Moreover, the productivity of this co-generation system will be increased through optimizing the carbon metabolic flux among lipid and gluconic acid synthesis pathways. This study will open the door to the possibility of engineering more co-generation systems for microbial lipid economical production.