NONRADIOACTIVE NUCLEIC ACID LABELING TECHNOLOGY

非放射性核酸标记技术

• 批准号: 3498924
• 负责人: MEAD M MCCABE
• 金额: $ 5万
• 依托单位: GENETIC VECTORS, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1994-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498924
• 关键词: guanidines; method development; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; oligonucleotides

Nucleic acid probes bearing detectable labels are widely used in the life sciences and biotechnology to provide a sensitive means to detect, localize, measure or amplify specific nucleic acid sequences in the course of genetic or clinical analyses and molecular biology manipulations. Genetic Vectors has developed a chemical procedure for incorporation of detectable labels into nucleic acids. It is capable of attaching virtually any detectable label to nucleic acids in a configuration that does not hinder Watson-Crick bonding. This new procedure has a variety of applications, from labeling probes and primers to measuring trace levels of nucleic acids. One of the primary commercial applications of the EpiDNA(TM) technology is its use for the preparation of labeled probes and primers by direct labeling of existing polynucleotides and oligonucleotides. In the studies outlined here, we will establish basic conditions for the rapid direct labeling of nucleic acids. The results of these studies will provide the basis for development of a line of commercial products, marketed under the trademark EpiDNA(TM), including nucleic acid assays, kits for preparation of labeled probes and primers, guanine derivatives for chemical and enzymatic synthesis of labeled nucleic acids, new derivatives of detectable molecules for labeling nucleic acids and pre-labeled probes for clinical and veterinary diagnostics.

携带可检测标记的核酸探针广泛用于生活中 科学和生物技术提供了一种灵敏的检测手段， 定位、测量或扩增特定的核酸序列 基因或临床分析和分子生物学操作。 遗传载体已经开发了一种化学方法， 将可检测的标记导入核酸中。它能够连接虚拟 核酸的任何可检测标记，其构型不 阻碍沃森-克里克键合。这种新的程序有各种各样的 从标记探针和引物到测量痕量水平 的核酸。的主要商业应用之一， EpiDNA（TM）技术用于制备标记探针， 通过直接标记现有多核苷酸的引物， 寡核苷酸在这里概述的研究中，我们将建立基础 快速直接标记核酸的条件。的结果 这些研究将为开发一系列 以商标EpiDNA（TM）销售的商业产品，包括 核酸测定，用于制备标记探针和引物的试剂盒， 用于化学和酶促合成标记的鸟嘌呤衍生物 核酸，标记用可检测分子的新衍生物 用于临床和兽医的核酸和预标记探针 诊断