NONRADIOACTIVE NUCLEIC ACID LABELING TECHNOLOGY

带有可检测标记的核酸探针在生活中被广泛使用 科学和生物技术提供了一种灵敏的手段来检测， 定位、测量或扩增过程中的特定核酸序列 基因或临床分析和分子生物学操作。 遗传载体已经开发出一种化学程序来结合 将可检测的标记转化为核酸。它能够虚拟地连接到 对构型中的核酸的任何可检测的标记 阻碍沃森-克里克的结合。这一新程序有各种不同的 应用，从标记探针和引子到测量痕量水平 关于核酸的。的主要商业应用之一 EpiDNA(TM)技术是其用于制备标记探针和 通过直接标记现有的多核苷酸和 寡核苷酸。在这里概述的研究中，我们将建立基本的 核酸快速直接标记的条件。结果是 这些研究将为开发一系列 商业产品，以EpiDNA(TM)商标销售，包括 核酸分析，标记探针和引物的制备试剂盒， 鸟嘌呤类化合物的化学合成和酶促合成 用于标记的新型可检测分子的衍生物--核酸 临床和兽医用核酸和预标记物探针 诊断。

Nucleic acid probes bearing detectable labels are widely used in the life sciences and biotechnology to provide a sensitive means to detect, localize, measure or amplify specific nucleic acid sequences in the course of genetic or clinical analyses and molecular biology manipulations. Genetic Vectors has developed a chemical procedure for incorporation of detectable labels into nucleic acids. It is capable of attaching virtually any detectable label to nucleic acids in a configuration that does not hinder Watson-Crick bonding. This new procedure has a variety of applications, from labeling probes and primers to measuring trace levels of nucleic acids. One of the primary commercial applications of the EpiDNA(TM) technology is its use for the preparation of labeled probes and primers by direct labeling of existing polynucleotides and oligonucleotides. In the studies outlined here, we will establish basic conditions for the rapid direct labeling of nucleic acids. The results of these studies will provide the basis for development of a line of commercial products, marketed under the trademark EpiDNA(TM), including nucleic acid assays, kits for preparation of labeled probes and primers, guanine derivatives for chemical and enzymatic synthesis of labeled nucleic acids, new derivatives of detectable molecules for labeling nucleic acids and pre-labeled probes for clinical and veterinary diagnostics.