CHEMICAL BASIS OF GROUP II INTRON FUNCTION

II组内含子功能的化学基础

• 批准号: 2640943
• 负责人: JULIANE K STRAUSS-SOUKUP
• 金额: $ 2.5万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-17 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2640943
• 关键词: RNA; RNA splicing; chemical structure function; genetic mapping; introns; nucleotide analog; ribozymes

The growing interest in RNA structure has stemmed from the discovery of catalytic RNA molecules that can fold to form active sites for catalysis of chemical reactions. Group II introns are self-splicing RNAs that are equipped to perform the two step transesterification reaction in both the forward and reverse directions. Reverse splicing by the intron can occur into a DNA or RNA substrate. With the assistance of an intron encoded maturase, the group II intron site-specifically inserts itself into duplex DNA. It is of interest to identify the RNA structural elements required for reverse splicing into DNA. Nucleotide Analog Interference Mapping (NAIM) is a novel chemogenetic approach to biochemically characterize RNA structure. After development of a reverse splicing assay, NAIM will be utilized to understand the chemical basis of group II intron structure and function. The interactions identified using NAIM will increase the understanding of group II intron structure and tertiary contacts at the atomic level. The study of group II intron splicing will yield numerous insights into pre-mRNA splicing, since these two processes are similar. High resolution details of the structure of the group II intron during reverse splicing may allow for development of novel site specific vectors that can be used as genetic agents for the treatment of cancer and viral infections.

对RNA结构的兴趣日益源于发现 催化RNA分子可以折叠以形成活性位点进行催化 化学反应。 II组内含子是自剪接的RNA 配备了在两者中进行两步式式反应 向前和反向。 内含子可以 发生在DNA或RNA底物中。 在内含子的协助下 编码成熟酶，II组内含子特定于插入自身 进入双工DNA。 识别RNA结构很有趣 反向剪接到DNA所需的元素。核苷酸类似物 干涉映射（NAIM）是一种新型的化学生成方法 生化表征RNA结构。 开发后 反剪接测定法，NAIM将用于了解化学物质 II组内含子结构和功能的基础。 交互 使用NAIM确定将增加对II组内含子的理解 原子水平的结构和三级接触。 小组的研究 II内含子剪接将产生大量的见解，以了解前MRNA剪接， 由于这两个过程相似。 高分辨率的细节 反剪接过程中II组内含子的结构可能允许 可以用作遗传的新型现场特定媒介的开发 治疗癌症和病毒感染的药物。