MINORITY PREDOCTORAL FELLOWSHIP

少数族裔博士前奖学金

• 批准号: 2403037
• 负责人: ANDREA R. MORRIS
• 金额: $ 2.87万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2403037
• 关键词: RNase protection assay; Urodela; cadherins; cell migration; embryogenesis; health science research support; histogenesis; in situ hybridization; integrins; nonmammalian vertebrate embryology; northern blottings; renal tubule; university student

Cell migration, the translocation of individual cells or whole tissue rudiments from one site in a developing embryo to another, is a critical component of morphogenesis. One very specific example of such active morphogenetic movements is seen in the development of the urogenital system. Pronephric duct cell migration in the axolotl, Ambystoma mexicanum, provides an excellent model system for studying the mechanisms controlling cell migration in vivo. Here, experiments are designed to identify, characterize and perform functional studies on specific cell surface molecules which have been implicated in the directed cell migration of A. mexicanum pronephric duct. These candidate molecules belong to two cell adhesion families, those representing cadherins and integrins. A PCR- based approach has been used to identify the expression of the candidate molecules during pronephric duct morphogenesis in the axolotl. The temporal and spatial expression of the candidates will then be assayed using Northern blotting, RNase protection assays and whole mount in situ hybridizations. Functional studies utilizing chromophore-assisted laser inactivation (CALI) will be used to inactivate each candidate molecule and determine whether its function is essential for normal pronephric duct cell migration.

细胞迁移，单个细胞或整个组织的移位 从发育中的胚胎的一个部位到另一个部位， 形态发生的组成部分。 一个非常具体的例子，这种积极的 在泌尿生殖系统的发育中可以看到形态发生运动 系统 钝口螈原肾管细胞的迁移 mexicanum为研究其作用机制提供了一个很好的模型系统 控制体内细胞迁移。 在这里，实验的目的是 对特定细胞进行鉴定、表征和功能研究 参与定向细胞迁移的表面分子 曲霉前肾管 这些候选分子属于两个 细胞粘附家族，代表钙粘蛋白和整联蛋白。 一个PCR- 的方法已被用来确定表达的候选人 分子在蝾螈前肾管形态发生。 的 然后分析候选物的时间和空间表达 使用北方印迹、RNA酶保护测定和原位整体封片 杂交 利用发色团辅助激光的功能研究 灭活（卡利）将用于鉴定每个候选分子， 确定其功能是否是正常前肾管细胞所必需的 迁移