MINORITY PREDOCTORAL FELLOWSHIP

少数族裔博士前奖学金

• 批准号: 2673390
• 负责人: ANDREA R. MORRIS
• 金额: $ 2.69万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2673390
• 关键词: RNase protection assay; Urodela; cadherins; cell migration; embryogenesis; health science research support; histogenesis; in situ hybridization; integrins; nonmammalian vertebrate embryology; northern blottings; renal tubule; university student

Cell migration, the translocation of individual cells or whole tissue rudiments from one site in a developing embryo to another, is a critical component of morphogenesis. One very specific example of such active morphogenetic movements is seen in the development of the urogenital system. Pronephric duct cell migration in the axolotl, Ambystoma mexicanum, provides an excellent model system for studying the mechanisms controlling cell migration in vivo. Here, experiments are designed to identify, characterize and perform functional studies on specific cell surface molecules which have been implicated in the directed cell migration of A. mexicanum pronephric duct. These candidate molecules belong to two cell adhesion families, those representing cadherins and integrins. A PCR- based approach has been used to identify the expression of the candidate molecules during pronephric duct morphogenesis in the axolotl. The temporal and spatial expression of the candidates will then be assayed using Northern blotting, RNase protection assays and whole mount in situ hybridizations. Functional studies utilizing chromophore-assisted laser inactivation (CALI) will be used to inactivate each candidate molecule and determine whether its function is essential for normal pronephric duct cell migration.

细胞迁移，单个细胞或整个组织的移位