MRNA EDITING PROTEINS--POSTTRANSCRIPTIONAL REGULATION OF HUMAN GENE EXPRESSION

mRNA编辑蛋白--人类基因表达的转录后调控

• 批准号: 2568990
• 负责人: A P PATTERSON
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2568990
• 关键词: amination; aminohydrolases; apolipoproteins; cytidine; enzyme biosynthesis; enzyme complex; gene expression; human genetic material tag; human tissue; messenger RNA; nucleotides; phosphorylation; posttranscriptional RNA processing; uridine; zinc

Our lab has focused on apoB mRNA editing as a model of post-transcriptional base modification in humans. ApoB mRNA editing is a site specific deamination of a cytidine residue to a uridine at nucleotide position 6666 in the ApoB mRNA transcript. The deamination reaction is a zinc dependent process mediated by a cytidine deaminase referred to as apolipoprotein B editing component 1 (APOBEC-1). RNA editing activity of APOBEC-1 is strictly dependent on assembly of an enzyme complex that includes APOBEC-1 (the catalytic subunit), and auxiliary proteins. During the past year, we have examined regulation of apoB mRNA editing by analysis of post-transcriptional protein phosphorylation.

我们的实验室一直专注于apoB mRNA的编辑，作为 人类转录后碱基修饰。ApoB mRNA编辑是 胞苷残基与尿苷的定点脱氨基反应 ApoB基因转录本中的6666位核苷酸。脱氨 反应是由胞苷脱氨酶介导的锌依赖过程。 称为载脂蛋白B编辑组件1(APOBEC-1)。核糖核酸 APOBEC-1的编辑活动严格依赖于一个 包括APOBEC-1(催化亚单位)的酶复合体，以及 辅助蛋白质。在过去的一年里，我们研究了监管 利用转录后蛋白分析进行载脂蛋白B的mRNA编辑 磷酸化。