MRNA EDITING PROTEINS--POSTTRANSCRIPTIONAL REGULATION OF HUMAN GENE EXPRESSION

mRNA编辑蛋白--人类基因表达的转录后调控

• 批准号: 6101252
• 负责人: A P PATTERSON
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6101252
• 关键词: amination; aminohydrolases; apolipoprotein B; cytidine; enzyme biosynthesis; enzyme complex; gene expression; human genetic material tag; human tissue; messenger RNA; nucleotides; phosphorylation; posttranscriptional RNA processing; uridine; zinc

Our lab has focused on apoB mRNA editing as a model of post-transcriptional base modification in humans. ApoB mRNA editing is a site specific deamination of a cytidine residue to a uridine at nucleotide position 6666 in the ApoB mRNA transcript. The deamination reaction is a zinc dependent process mediated by a cytidine deaminase referred to as apolipoprotein B editing component 1 (APOBEC-1). RNA editing activity of AP0BEC-l is strictly dependent on assembly of an enzyme complex that includes APOBEC-l (the catalytic subunit), and auxiliary proteins. During the past year, we have examined regulation of apoB mRNA editing by analysis of post-transcriptional protein phosphorylation.

我们的实验室专注于apoB mRNA编辑作为一种模型， 人类的转录后碱基修饰。ApoB mRNA编辑 胞苷残基位点特异性脱氨为尿苷， ApoB mRNA转录物中的核苷酸位置6666。脱氨基 反应是由胞苷脱氨酶介导的锌依赖性过程 称为载脂蛋白B编辑组分1（APOBEC-1）。RNA AP 0 BEC-I的编辑活性严格依赖于 包括APOBEC-1（催化亚基）的酶复合物，和 辅助蛋白在过去一年，我们研究了 通过转录后蛋白分析进行apoB mRNA编辑 磷酸化