MRNA EDITING PROTEINS--POSTTRANSCRIPTIONAL REGULATION OF HUMAN GENE EXPRESSION

mRNA编辑蛋白--人类基因表达的转录后调控

• 批准号: 6161312
• 负责人: A P PATTERSON
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6161312
• 关键词: amination; aminohydrolases; apolipoproteins; cytidine; enzyme biosynthesis; enzyme complex; gene expression; human genetic material tag; human tissue; messenger RNA; nucleotides; phosphorylation; posttranscriptional RNA processing; uridine; zinc

Our lab has focused on apoB mRNA editing as a model of post- transcriptional base modification in humans. ApoB mRNA editing is a site specific deamination of a cytidine residue to a uridine at nucleotide position 6666 in the ApoB mRNA transcript. The deaminination reaction is a zinc dependent process mediated by a cytadine deaminase referred to as apolipoprotein B editing component 1 (APOBEC-1), RNA editing activity of APOBEC-1 is trictly dependent on assembly on an enzyme complex that includes APOBEC-1(the catalytic subunit), and auxiliary proteins. During the past year, we have examined regulation of apoB mRNA editing by analysis of post-transcriptional proetin phosphorylation.

我们的实验室专注于apoB mRNA编辑作为一个模型， 在人类中的转录碱基修饰。ApoB mRNA编辑是 胞苷残基在核苷酸处特异性脱氨为尿苷 ApoB mRNA转录物中的位置6666。脱氨反应是 由cytadine脱氨酶介导的锌依赖性过程， 载脂蛋白B编辑组分1（APOBEC-1）， APOBEC-1严格依赖于在酶复合物上的组装， 包括APOBEC-1（催化亚基）和辅助蛋白。期间 在过去的一年里，我们研究了apoB mRNA编辑的调节， 转录后蛋白磷酸化的分析。