SYNTHESIS AND RELEASE OF BIOGENIC AMINES

这些研究集中在酪氨酸的调节特性 羟化酶、色氨酸羟化酶及其辅因子 四氢生物蝶呤（BH 4）在生物合成和释放 脑中的儿茶酚胺和血清素 我们首次成功克隆了人脑色氨酸羟化酶， 步骤来表征其监管特性。 我们已经从大鼠和牛脑中纯化了14-3-3蛋白， 研究了14-3-3在人重组 色氨酸羟化酶（与麦芽糖结合蛋白融合）， E.coli. 融合色氨酸羟化酶磷酸化， cAMP依赖性蛋白激酶和钙调蛋白依赖性蛋白 激酶II。 初步结果表明， 羟化酶通过与14-3-3温育来活化。 通过使用 表面等离子体共振光谱，14-3-3和 色氨酸羟化酶已得到证实。

These studies have focused on the regulatory properties of tyrosine hydroxylase, tryptophan hydroxylase, and their cofactor tetrahydrobiopterin (BH4) in the biosynthesis and release of catecholamine and serotonin in the brain. We have successfully cloned human brain tryptophan hydroxylase as a first step to characterizing its regulatory properties. We have purified the 14-3-3 protein from rat and bovine brains and investigated the role of 14-3-3 in the regulation of human recombinant tryptophan hydroxylase (fused with maltose binding protein) expressed in E.coli. Fusion tryptophan hydroxylase was phosphorylated in the presence of both cAMP-dependent protein kinase and calmodulin-dependent protein kinase II. Preliminary results indicate that the phosphorylated hydroxylase is activated by incubation with 14-3-3. Through the use of surface plasmon resonance spectroscopy, tight binding between 14-3-3 and tryptophan hydroxylase has been demonstrated.