EFFECT OF REST ON HUMAN PROLACTIN SECRETION

休息对人类催乳素分泌的影响

• 批准号: 2447831
• 负责人: T A WEHR
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2447831
• 关键词: clinical research; human subject; prolactin; rest; secretion; sleep; speech

Human prolactin secretion rises dramatically at night. For more than 20 years, this rise has been considered to be sleep-dependent, because it usually coincides with sleep, it can be delayed by delaying sleep and it can be clocked by preventing sleep. Our recent work suggests, however, that the nighttime rise of PRL secretion is triggered by quiet rest, rather than sleep. The purpose of the present study is to attempt to confirm this preliminary finding in a controlled experiment in which effects on PRL secretion of undisturbed rest, and of rest disturbed conversation, will be compared in individuals lying in bed in the dark. The results of the study may prove to be relevant to the neurobiology of psychiatric syndromes, many of which are characterized by an absence or an excess of quiescence and the tranquility. Since animal research has already shown that PRL can induce quiescent states, it is also possible that pharmacological manipulations of PRL or PRL receptors might prove useful as treatments for these syndromes. More generally, confirmation that undisturbed rest stimulates PRL secretion would open up a new area of human behavioral neuroendocrinology whose relevance to human health and well-being could be a focus for further investigation. Subjects are admitted to an inpatient unit of the NIH Clinical Center for four consecutive nights. Each night, from 6:00 pm to 8:00 am, they are asked to lie in bed in a dark room and to rest and sleep (our imposition of this schedule is simply a device that allows our subjects to remain awake in the dark without trying to do so and enables us to dissect away effects of sleep from the effects of undisturbed rest on PRL secretion). On Night 3 or 4, as determined by balanced-order random assignment, they are asked to engage in conversation with a staff member during the first half hour of the dark period (6:00 pm to 6:30 pm) while lying in bed in the dark room. On Night 1 (an adaptation night) and on both Nights 3 and 4, an intravenous catheter is inserted in a forearm vein and blood samples are drawn at 5:30 pm -(30 min), 6:00 pm (0 min) and 6:30 pm (+30 min). Later, levels of PRL are measured in plasma obtained from the samples obtained on Nights 3 and 4. On the basis of our preliminary findings, we expect that +30 PRL levels, relative to -30 min and 0 min baseline levels, will increase in the Rest (no conversation) Condition but not in the Disturbed Rest (conversation) Condition.

人的催乳素分泌在夜间急剧上升。了20多 多年来，这种上升一直被认为是睡眠依赖的，因为它 通常与睡眠相吻合，它可以通过延迟睡眠来延迟， 可以通过阻止睡眠来计时。然而，我们最近的研究表明， 夜间PRL分泌的增加是由安静的休息引起的， 而不是睡觉。本研究的目的是试图 在一项对照实验中证实了这一初步发现， 不受干扰的休息和受干扰的休息对PRL分泌的影响 谈话，将比较个人躺在床上在黑暗中。 这项研究的结果可能与神经生物学有关。 精神病综合征，其中许多的特点是缺乏或 过度的安静和宁静。由于动物研究已经 已经表明PRL可以诱导静止状态，也有可能 对催乳素或催乳素受体的药理学操作可能证明 可用于治疗这些综合症。一般来说，确认 不受干扰的休息会刺激PRL分泌， 人类行为神经内分泌学与人类健康的关系 和幸福感可能是进一步调查的重点。受试者 入住NIH临床中心的住院部， 连续的夜晚。每天晚上6点到早上8点， 躺在床上，在黑暗的房间里，休息和睡眠（我们的强加 这个时间表只是一个让我们的实验对象保持清醒的装置 在黑暗中没有试图这样做，使我们能够解剖了 睡眠对PRL分泌的影响）。 在第3或第4天晚上，根据平衡顺序随机分配的结果， 被要求在第一次会议期间与一名工作人员交谈， 半小时的黑暗期（下午6时至6时30分），而躺在床上， 黑暗的房间。在第一晚（改编之夜）和第三晚， 4、将静脉导管插入前臂静脉， 在下午5：30-（30分钟）、下午6：00（0分钟）和下午6：30（+30分钟）取样 min）。随后，测量从受试者获得的血浆中的PRL水平。 在第3和第4天晚上采集的样本。根据我们初步的 结果，我们预计+30 PRL水平，相对于-30分钟和0分钟， 基线水平，将在休息（无对话）条件下增加 但不是在干扰休息（谈话）条件下。