EFFECT OF REST ON HUMAN PROLACTIN SECRETION

人的催乳素分泌量在夜间急剧上升。已经超过20年了 多年来，这种上升一直被认为是睡眠依赖的，因为它 通常与睡眠时间重合，可以通过延迟睡眠来延迟，而且它 可以通过防止睡眠来计时。然而，我们最近的研究表明， 夜间催乳素分泌的上升是由安静的休息引发的， 而不是睡觉。本研究的目的是试图 在一项对照实验中证实了这一初步发现 安静状态下和安静状态下对催乳素分泌的影响 交谈，会被比作躺在床上的黑暗。 这项研究的结果可能被证明与神经生物学有关 精神综合症，其中许多的特征是缺席或 一种过度的宁静和宁静。因为动物研究已经 已经证明PRL可以诱导静止状态，这也是可能的 PRL或PRL受体的药理操作可能证明 对这些综合症的治疗很有用。更广泛地说，确认 不受干扰的休息刺激催乳素分泌将开辟一个新的领域 与人类健康相关的人类行为神经内分泌学 而幸福感可能是进一步调查的重点。主题是 在NIH临床中心的住院部住了四年 连续几个晚上。每晚，从下午6点到早上8点，他们被要求 在黑暗的房间里躺在床上休息和睡觉(我们强加的 这个时间表只是一个让我们的受试者保持清醒的装置 在黑暗中，而不是试图这样做，使我们能够解剖 不受干扰的休息对PRL分泌的影响)。 在第三夜或第四夜，由平衡顺序随机分配确定，他们 被要求在第一次面试中与一名工作人员交谈 躺在床上的半小时黑暗时期(下午6：00至6：30) 黑暗的房间。在第一夜(改编之夜)和第三夜和第三夜 4、将静脉导管插入前臂静脉和血液中 抽样时间为下午5：30-30分钟、下午6：00(0分钟)和下午6：30(30分钟) Min)。随后，测量血浆中催乳素的水平 在第三晚和第四晚获得的样本。根据我们初步的 结果，我们预计30个PRL水平，相对于-30分钟和0分钟 基线水平，在休息(无对话)条件下会增加 但不是在受到干扰的休息(交谈)状态下。

Human prolactin secretion rises dramatically at night. For more than 20 years, this rise has been considered to be sleep-dependent, because it usually coincides with sleep, it can be delayed by delaying sleep and it can be clocked by preventing sleep. Our recent work suggests, however, that the nighttime rise of PRL secretion is triggered by quiet rest, rather than sleep. The purpose of the present study is to attempt to confirm this preliminary finding in a controlled experiment in which effects on PRL secretion of undisturbed rest, and of rest disturbed conversation, will be compared in individuals lying in bed in the dark. The results of the study may prove to be relevant to the neurobiology of psychiatric syndromes, many of which are characterized by an absence or an excess of quiescence and the tranquility. Since animal research has already shown that PRL can induce quiescent states, it is also possible that pharmacological manipulations of PRL or PRL receptors might prove useful as treatments for these syndromes. More generally, confirmation that undisturbed rest stimulates PRL secretion would open up a new area of human behavioral neuroendocrinology whose relevance to human health and well-being could be a focus for further investigation. Subjects are admitted to an inpatient unit of the NIH Clinical Center for four consecutive nights. Each night, from 6:00 pm to 8:00 am, they are asked to lie in bed in a dark room and to rest and sleep (our imposition of this schedule is simply a device that allows our subjects to remain awake in the dark without trying to do so and enables us to dissect away effects of sleep from the effects of undisturbed rest on PRL secretion). On Night 3 or 4, as determined by balanced-order random assignment, they are asked to engage in conversation with a staff member during the first half hour of the dark period (6:00 pm to 6:30 pm) while lying in bed in the dark room. On Night 1 (an adaptation night) and on both Nights 3 and 4, an intravenous catheter is inserted in a forearm vein and blood samples are drawn at 5:30 pm -(30 min), 6:00 pm (0 min) and 6:30 pm (+30 min). Later, levels of PRL are measured in plasma obtained from the samples obtained on Nights 3 and 4. On the basis of our preliminary findings, we expect that +30 PRL levels, relative to -30 min and 0 min baseline levels, will increase in the Rest (no conversation) Condition but not in the Disturbed Rest (conversation) Condition.