MOLECULAR BASIS OF TRANSCRIPTIONAL ACTIVATION BY TFIIIA

TFIIIA 转录激活的分子基础

• 批准号: 2749965
• 负责人: MARTYN K DARBY
• 金额: $ 15.44万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2749965
• 关键词: DNA directed RNA polymerase; Xenopus; binding proteins; gene expression; intermolecular interaction; molecular cloning; protein structure function; ribosomal RNA; transcription factor; western blottings

DESCRIPTION: In this proposal, Dr. Darby describes experiments focused on the mechanisms involved in the recruitment of the transcriptional machinery to a 5S rRNA gene by the specific factor TFIIIA. A protein has been identified, TIP21 which interacts with the C-terminus of TFIIIA. This protein has homology with individual subunits of both yeast RNA polymerase II and III. Dr. Darby wishes to test any functional role of TIP21 in 5S rRNA gene transcription in vitro and in vivo using standard approaches (immunodepletion, ribozyme ablation) and to examine structure-function (Far-Western, two hybrid).

产品说明： 在这个提议中，达比博士描述了 重点是参与征聘的机制 转录机器的5S rRNA基因的特定因素 TFIIIA。 已经鉴定了一种蛋白质，TIP 21，其与 TFIIIA的C末端。 该蛋白质与人源性前列腺素具有同源性， 酵母RNA聚合酶II和III的亚基。 达比医生希望 测试TIP 21在5SrRNA基因转录中任何功能作用 体外和体内使用标准方法（免疫耗竭， 核酶切除）并检查结构-功能（Far-Western， 两个混合物）。