REPLICATION PROTEIN A AND CELL CYCLE REGULATION

描述：(改编自提案)：提案建议 RPA是一种多亚基(p70、p34和p11)蛋白质，与DNA有关 复制和DNA修复，可能是调控的目标。这根茎 由于RPA亚单位的数量在 细胞周期。因此，RPA的DNA复制和DNA修复活动 可以由蛋白质组装或翻译后调控 修改。 调查人员建议测试RPA复合体的组装 在细胞周期中受到调节。此外，他还将审查 RPA是否调节SV40起始复合体的形成 复制的来源。因为RPA的磷酸化可能会起到 在规范其活动时，调查员将绘制 磷酸化位点，并将表征该蛋白中的激酶 磷酸化。RPA磷酸化位点将发生突变和突变 RPA将与野生型RPA一起在偶联修复中进行分析- 复制系统，这是最近在研究人员的 实验室。

DESCRIPTION: (adapted from the proposal): The proposal suggests that RPA, a multisubunit (p70, p34 and p11) protein, which is involved in DNA replication and DNA repair, may be a target for regulation. This stems from the fact that the amount of RPA subunits does not vary during the cell cycle. Hence, the DNA replication and DNA repair activities of RPA could be regulated by either protein assembly or post- translational modification. The investigator proposes to test whether the assembly of the RPA complex is regulated during the cell cycle. In addition, he will examine whether RPA regulates the formation of initiation complexes at the SV40 origin of replication. Since phosphorylation of RPA could have a role in regulating its activities, the investigator will map the phosphorylation sites and will characterize the kinases in this phosphorylation. RPA phosphorylation sites will be mutated and mutant RPA will be analyzed along with the wild type RPA in a coupled repair- replication system, which was recently developed in the investigator's laboratory.