REPLICATION PROTEIN A AND CELL CYCLE REGULATION

说明：（改编自提案）：提案建议， RPA是一种多亚基（p70、p34和p11）蛋白，参与DNA合成， 复制和DNA修复可能是调控的目标。这源自 由于RPA亚基的量在整个过程中不变化， 细胞周期 因此，RPA的DNA复制和DNA修复活性 可以通过蛋白质组装或翻译后调节 改性 研究人员建议测试RPA复合物的组装是否 在细胞周期中受到调节。 此外，他还将审查 RPA是否调节SV 40处起始复合物的形成 复制起点 由于RPA的磷酸化可能在 在管理其活动时，调查人员将绘制 磷酸化位点，并将表征激酶在这个 磷酸化 RPA磷酸化位点会发生突变， RPA将与野生型RPA一起沿着在偶联修复中进行分析- 复制系统，这是最近开发的研究人员的 实验室

DESCRIPTION: (adapted from the proposal): The proposal suggests that RPA, a multisubunit (p70, p34 and p11) protein, which is involved in DNA replication and DNA repair, may be a target for regulation. This stems from the fact that the amount of RPA subunits does not vary during the cell cycle. Hence, the DNA replication and DNA repair activities of RPA could be regulated by either protein assembly or post- translational modification. The investigator proposes to test whether the assembly of the RPA complex is regulated during the cell cycle. In addition, he will examine whether RPA regulates the formation of initiation complexes at the SV40 origin of replication. Since phosphorylation of RPA could have a role in regulating its activities, the investigator will map the phosphorylation sites and will characterize the kinases in this phosphorylation. RPA phosphorylation sites will be mutated and mutant RPA will be analyzed along with the wild type RPA in a coupled repair- replication system, which was recently developed in the investigator's laboratory.