PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
批准号:
2749943
负责人:
JOSE-ANGEL CONCHELLO
金额:
$17.41万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-01 至 1999-07-31
中文摘要
共焦扫描显微镜(CSM)现在广泛用于三个-
固定标本的三维(3D)可视化。然而,生活
样品经常被高强度激光损坏和/或漂白
CSM所需的激发光。一个常见的解决方案是打开
共焦光圈轻微,从而允许更多的光到达
检测器然而,更大的共焦孔径也接受更多的非共焦。
聚焦光线并降低CSM的深度分辨率。 丢失的
可以使用计算反卷积算法来恢复分辨率。
即使是严格的共焦显微镜也受益于增加的
解卷积算法提供的分辨率。大多数调制解调器CSM
配有一台主机,可以将共焦图像的3D堆栈数字化。
尽管计算机的可用性,共焦图像的解卷积
还没有被广泛使用。其中一个关键原因是,
反卷积算法需要点扩展的准确知识,
描述显微镜的函数(PSF)。 在现代CSM中,
PSF的测量或计算充其量是困难的。在这些
在某些情况下,需要估计PSF和样品荧光
分布同时从图像,一种方法称为盲
解卷积(BD)。BD的当前方法是缓慢的,因为它们
逐点估计PSF。
这项研究的长期目标是提供更快、更多的
鲁棒的计算反卷积算法,
测量或计算显微镜的PSF。
为了实现这一目标,使用PSF的物理物理模型
这取决于少量的未知参数,然后估计
将派生方法来同时获得这些参数的值
与标本荧光分布。虽然长期目标
是BD的部分共焦图像,非共焦显微镜用户将
他们也从这项研究的结果中受益。
英文摘要
Confocal scanning microscopes (CSM) are now widely used for three-
dimensional (3D) visualization of fixed specimens. However, living
specimens are often damaged and/or bleached by the high intensity laser
excitation light required by the CSM. A common solution is to open the
confocal aperture slightly and thus allow more light to reach the
detector. A larger confocal aperture, however, accepts also more out-of-
focus light and degrades the depth resolution of the CSM. The lost
resolution can be recovered using computational deconvolution algorithms.
Even the strictly confocal microscope benefits from the increased
resolution that deconvolution algorithms provide. Most of the modem CSM's
come with a host computer that digitizes 3D stacks of confocal images.
Despite the availability of a computer, deconvolution of confocal images
has not been widely used. One of the key reasons is that most
deconvolution algorithms require accurate knowledge of the point-spread-
function (PSF) that describes the microscope. In modern CSM's, the
measurement or computation of the PSF is difficult at best. In these
cases, it is necessary to estimate the PSF and the specimen fluorescence
distribution simultaneously from the image, an approach called blind
deconvolution (BD). Current methods for the BD are slow because they
estimate the PSF point by point.
The long term goal of the research proposed is to provide faster and more
robust computational deconvolution algorithms that do not require
measuring or computing the microscope's PSF.
To achieve this goal, a mathematical-physical model for the PSF is used
that depends on a small number of unknown parameters, then estimation
methods will be derived to obtain the values of these parameters together
with the specimen fluorescent distribution. Although the long term goal
is BD of partially confocal images, nonconfocal microscope users will
also benefit from the results of this research.
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会议论文
COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2910317
-
项目类别:
-
资助金额:$19.77万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6682927
-
项目类别:
-
资助金额:$26.48万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2701827
-
项目类别:
-
资助金额:$19.37万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6796225
-
项目类别:
-
资助金额:$24.48万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2024260
-
项目类别:
-
资助金额:$23.13万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6473523
-
项目类别:
-
资助金额:$28.52万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
-
批准号:2187334
-
项目类别:
-
资助金额:$14.72万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2907740
-
项目类别:
-
资助金额:$22.45万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
-
批准号:3308955
-
项目类别:
-
资助金额:$14.25万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:6180213
-
项目类别:
-
资助金额:$22.26万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
3D RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
-
批准号:2187335
-
项目类别:
-
资助金额:$12.25万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2187336
-
项目类别:
-
资助金额:$19.76万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2459497
-
项目类别:
-
资助金额:$17.08万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:6385829
-
项目类别:
-
资助金额:$22.3万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
海外基金