PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
批准号:
2749943
负责人:
JOSE-ANGEL CONCHELLO
金额:
$17.41万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-01 至 1999-07-31
中文摘要
共聚焦扫描显微镜(CSM)目前已广泛应用于三维扫描
英文摘要
Confocal scanning microscopes (CSM) are now widely used for three-
dimensional (3D) visualization of fixed specimens. However, living
specimens are often damaged and/or bleached by the high intensity laser
excitation light required by the CSM. A common solution is to open the
confocal aperture slightly and thus allow more light to reach the
detector. A larger confocal aperture, however, accepts also more out-of-
focus light and degrades the depth resolution of the CSM. The lost
resolution can be recovered using computational deconvolution algorithms.
Even the strictly confocal microscope benefits from the increased
resolution that deconvolution algorithms provide. Most of the modem CSM's
come with a host computer that digitizes 3D stacks of confocal images.
Despite the availability of a computer, deconvolution of confocal images
has not been widely used. One of the key reasons is that most
deconvolution algorithms require accurate knowledge of the point-spread-
function (PSF) that describes the microscope. In modern CSM's, the
measurement or computation of the PSF is difficult at best. In these
cases, it is necessary to estimate the PSF and the specimen fluorescence
distribution simultaneously from the image, an approach called blind
deconvolution (BD). Current methods for the BD are slow because they
estimate the PSF point by point.
The long term goal of the research proposed is to provide faster and more
robust computational deconvolution algorithms that do not require
measuring or computing the microscope's PSF.
To achieve this goal, a mathematical-physical model for the PSF is used
that depends on a small number of unknown parameters, then estimation
methods will be derived to obtain the values of these parameters together
with the specimen fluorescent distribution. Although the long term goal
is BD of partially confocal images, nonconfocal microscope users will
also benefit from the results of this research.
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COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2910317
-
项目类别:
-
资助金额:$19.77万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6682927
-
项目类别:
-
资助金额:$26.48万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6796225
-
项目类别:
-
资助金额:$24.48万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2701827
-
项目类别:
-
资助金额:$19.37万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
Computational Optical Sectioning Microscopy Algorithms
-
批准号:6473523
-
项目类别:
-
资助金额:$28.52万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
COMPUTATIONAL OPTICAL SECTIONING MICROSCOPY ALGORITHMS
-
批准号:2024260
-
项目类别:
-
资助金额:$23.13万
-
财政年份:1997
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
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批准号:2187334
-
项目类别:
-
资助金额:$14.72万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2907740
-
项目类别:
-
资助金额:$22.45万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
-
批准号:3308955
-
项目类别:
-
资助金额:$14.25万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:6180213
-
项目类别:
-
资助金额:$22.26万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:6385829
-
项目类别:
-
资助金额:$22.3万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2187336
-
项目类别:
-
资助金额:$19.76万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
3D RECONSTRUCTION OF PARTIALLY CONFOCAL IMAGES
-
批准号:2187335
-
项目类别:
-
资助金额:$12.25万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
PARAMETRIC BLIND DECONVOLUTION OF MICROSCOPIC IMAGES
-
批准号:2459497
-
项目类别:
-
资助金额:$17.08万
-
财政年份:1993
-
负责人:JOSE-ANGEL CONCHELLO
-
依托单位:
海外基金