CORE--MOLECULAR ANALYSIS

核心C的目的是为 本计划项目中的项目调查员。免疫印迹法， Northern和原位杂交分析(以确定 IL-1、S100β和β-APP及其编码的mRNA以及其他 可能感兴趣的蛋白质，例如转化生长因子-β和成纤维细胞生长因子)将是 可根据要求提供；还将提供S100测试版和GFAP ELISA 用于大量样品的快速筛选。总的水平 蛋白质、总核糖核酸和核糖体核糖核酸将作为常规检查 确定特定目标相对水平所需的分析 提到的蛋白质和mRNAs。将被分析用于研究的人体组织 将由项目调查员从 神经病理学核心(B1和B2)。动物组织或细胞将来自 项目2和项目3的实验中使用的大鼠。分析数据 在此核心中执行的工作将作为以下形式提供给项目调查员 包括原始数据和初步分析数据。例如，来自 北部和西部的免疫印迹分析将返回到 调查员作为原始数据(转移的凝胶和胶片的图片)和 纳克/微克组织或纳克/微克 提取。来自ELISA的数据将以原始数据的形式返回 每个样品的光密度单位，以及标准曲线 同时产生，并计算ng/微克蛋白质和 Ng/微克提取物。在原位杂交后，切片将 返回给调查员或送到核心D进行分析。数据来自 培养物的原位杂交将被退还给调查人员 作为黑石物理物理服务器/井。人类和动物组织研究的价值将是 已输入相关数据库。

The purpose of Core C is to provide molecular analysis services to the Project investigators in this Program Project. Western immunoblot, northern and in situ hybridization analyses (to determine the levels of IL-1, S100beta, and beta-APP and their encoding mRNAs, as well as other proteins that may be of interest, e.g., TGF-beta, and FGF) will be available upon request; S100beta and GFAP ELISAs will also be available for rapid screening of large numbers of samples. The levels of total protein, total RNA, and ribosomal RNA will be performed as routine analyses necessary for determining the relative levels of the specific proteins and mRNAs mentioned. The human tissue to be analyzed for studies in Project 1 will have been obtained by Project investigators from the Neuropathology Core (B1 and B2). The animal tissue or cells will be from rats used in experiments in Projects 2 and 3. Data from analyses performed in this Core will be provided to the Project Investigators as both raw data and preliminarily analyzed data. For example, data from northern and western immunoblot analyses will be returned to the investigator as raw data (pictures of gels and films of transfers) and ng/micrograms tissue or ng/micrograms of extract. Data from ELISAs will be returned as raw data in the form of optical density units per sample, together with a standard curve generated at the same time, and calculation of ng/micrograms protein and ng/micrograms extract. Following in situ hybridization, sections will be returned to the investigator or sent to Core D for analysis. Data from in situ hybridization of cultures will be returned to the investigator as cpm/well. Values from studies of human and animal tissue will be entered into the relevant database.