CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
基本信息
- 批准号:2628346
- 负责人:
- 金额:$ 26.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1993
- 资助国家:美国
- 起止时间:1993-05-01 至 2002-04-30
- 项目状态:已结题
- 来源:
- 关键词:Schizosaccharomyces pombe biological signal transduction cell cycle enzyme activity fungal genetics gene complementation gene expression gene interaction guanosinetriphosphatases immunoelectron microscopy laboratory rabbit molecular cloning phenotype protein structure function southern blotting temperature sensitive mutant western blottings
项目摘要
The long term objective of the proposed research is to elucidate the
mechanism by which the morphological changes required for the mitosis
to interphase transition are regulated. Because of its excellent
genetics and cytology, fission yeast is an excellent experimental system
in which to study this point in the cell cycle at which the chromosomes
decondense, the nuclear envelope reforms around the chromosomes, and the
cells undergo cytokinesis and separate from one another. The focus of
this proposal is on spi1, the evolutionarily conserved GTPase that is
essential for the mitosis to interphase transition in fission yeast.
spi1 is nearly identical to the human proto-oncogene p21ras in its GTP
binding and hydrolysis domains, and its regulators are also
evolutionarily conserved. The proposed studies will further our
understanding of the function of this eukaryotic GTPase in particular,
and the findings will be applicable to studies of the large family of
structurally related GTPases that regulate a wide variety of cellular
functions, and to the elucidation of the mechanism by which GTPases
interact with their downstream targets via the activation of signal
transduction pathways. Perturbations in the GTPase cycle, leading to an
accumulation of either the GTP- or GDP-bound forms, have similar, easily
monitored phenotypic consequences. This observation led to the
generation of a collection of genes and mutants that based on a variety
of criteria are likely to identify regulators and downstream targets of
the spi1 GTPase. The proposed genetic, phenotypic, and biochemical
analyses will elucidate their relationship to the known components of
the spi1 GTPase system. The Specific Aims of this proposal are: SPECIFIC
AIM I. Identify targets of the spi1 GTPase by characterizing the sns
mutants and the med genes that interact genetically with components of
the GTPase system; SPECIFIC AIM II. Identify targets of the spi1 GTPase
by isolating genes that when overproduced can rescue the temperature
sensitive lethality of the pim1-d1 mutuant; and SPEICIFIC AIM III. Test
the hypothesis that the nuclear envelope is a downstream target of the
spi1 GTPase system.
拟议研究的长期目标是阐明
有丝分裂所需的形态变化的机制
到相间转变是受调节的。因为它的卓越性能
遗传学和细胞学,分裂酵母是一个很好的实验系统
研究细胞周期中的这个点,在这个点上,染色体
解聚,核膜在染色体周围重塑,而
细胞经历胞质分裂并彼此分离。的关注点
这一建议是在sp1上提出的,sp1是一种进化上保守的GTP酶
是分裂酵母有丝分裂向间期转变所必需的。
Sp1基因与人类原癌基因p21ras的gtp几乎完全相同。
结合和水解区,及其调节因子也是
进化上保守的。拟议的研究将进一步推动我们的
尤其是对这种真核细胞GTP酶的功能的理解,
这些发现将适用于对大家族的研究。
结构上相关的GTP酶,调节多种细胞
功能,并有助于阐明GTP酶的机制
通过激活信号与其下游目标相互作用
转导通路。GTPase循环中的扰动,导致
GTP或GDP绑定形式的积累很容易具有类似的
监测表型后果。这一观察结果导致了
基于品种的基因和突变体集合的生成
的标准很可能确定监管机构和下游目标
Sp1 GTP酶。建议的遗传、表型和生化
分析将阐明它们与已知成分的关系
Sp1 GTPase系统。这项建议的具体目标是:具体
目的I.通过鉴定SNS鉴定Spi1 GTPase的靶点
突变体和与基因成分相互作用的MED基因
GTP酶系统;特异性AIM II.鉴定Spi1 GTP酶的靶标
通过分离当过度生产时可以拯救温度的基因
Pim1-d1突变体的敏感致死性及SPEICIFIC AIM III试验
核包膜是核膜下游靶子的假设
Sp1 GTPase系统。
项目成果
期刊论文数量(0)
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{{ truncateString('SHELLEY SAZER', 18)}}的其他基金
NUCLEAR SHAPE DURING MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂过程中的核形状
- 批准号:
7355020 - 财政年份:2006
- 资助金额:
$ 26.86万 - 项目类别:
ROLES OF TWO IMPORTIN-ALPHA ISOFORMS IN FISSION YEAST
裂殖酵母中两种输入α异构体的作用
- 批准号:
6979592 - 财政年份:2004
- 资助金额:
$ 26.86万 - 项目类别:
THE ROLES OF IMPORTIN-ALPHA ISOFORMS IN FISSION YEAST
输入-α异构体在裂殖酵母中的作用
- 批准号:
6979607 - 财政年份:2004
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
2186660 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
2910119 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
6585712 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
2415188 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
6385801 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
CONTROL OF THE EXIT FROM MITOSIS IN FISSION YEAST
裂殖酵母有丝分裂退出的控制
- 批准号:
2186661 - 财政年份:1993
- 资助金额:
$ 26.86万 - 项目类别:
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