STRUCTURE AND FUNCTION OF THE INTEGRIN ALPHA 1 BETA 1
整合素 ALPHA 1 BETA 1 的结构和功能
基本信息
- 批准号:2734661
- 负责人:
- 金额:$ 25.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1990
- 资助国家:美国
- 起止时间:1990-07-01 至 1999-06-30
- 项目状态:已结题
- 来源:
- 关键词:3T3 cells CHO cells cell cell interaction cell migration chimeric proteins collagen crosslink extracellular matrix human tissue integrins molecular cloning nucleic acid probes platelet activation protein structure function receptor binding receptor coupling receptor expression site directed mutagenesis transfection /expression vector
项目摘要
Our research is focused on the structure and function of integrins, which
are transmembrane heterodimeric receptors for a number of extracellular
matrix molecules. These receptors are composed of alpha and beta subunits.
Initially, we have used the human integrin alpha1beta1 as a model system in
order to answer major questions of the structure-function relationships in
alpha and beta domains. We have recently shown that both cytoplasmic
domains of these receptors may be controlling integrin intracellular
function. The goal of this proposal is to determine the relationship
between the structure of integrin receptors and their functions. We have
two primary broad goals in this project. One, we will characterize the
mechanism by which post-ligand binding events in integrins occur, and
isolate genes which mediate those events. Two, we will identify ligand
binding domains, and then isolate genes involved in the modulation of the
affinity of ligand binding. Our recent structure-function experiments have
shown that the alpha cytoplasmic domain plays a regulatory role in the
modulation of integrin beta cytoplasmic function. It accomplishes this via
an association between these two domains, which we are studying by site-
directed mutagenesis of both domains. We will use a potent dominant
negative construct as a probe for the identification of beta cytoplasmic
domain binding proteins, through a strategy which involves rescue of the
dominant negative phenotype. Our experiments identifying ligand binding
motifs within alpha1 continue, and we will pinpoint regions necessary for
collagen IV binding. Lastly, we will study activation of integrins using
the platelet integrin IIb/IIIa, which is then expressed in CHO cells.
Using a newly developed permeabilization scheme, we will be able to
identify substances required for inside out activation. These experiments
will help us understand regulation of adhesion, which is central to
migration in atherosclerosis. Furthermore, these experiments will also aid
our understanding of the mechanism of integrin activation via inside-out
signalling, which is critical in migration and in platelet adhesion.
我们的研究重点是整合素的结构和功能
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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EUGENE E. MARCANTONIO其他文献
EUGENE E. MARCANTONIO的其他文献
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{{ truncateString('EUGENE E. MARCANTONIO', 18)}}的其他基金
Ph 2a Study of S48168 (ARM210) for CPVT 1 IND 152773 (09/11/2020)
S48168 (ARM210) CPVT 1 IND 152773 的 Ph 2a 研究 (09/11/2020)
- 批准号:
10280877 - 财政年份:2021
- 资助金额:
$ 25.73万 - 项目类别:
Ph 2a Study of S48168 (ARM210) for CPVT 1 IND 152773 (09/11/2020)
S48168 (ARM210) CPVT 1 IND 152773 的 Ph 2a 研究 (09/11/2020)
- 批准号:
10492470 - 财政年份:2021
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
2697501 - 财政年份:1998
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
6534088 - 财政年份:1998
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
2887510 - 财政年份:1998
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
6373666 - 财政年份:1998
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
6170957 - 财政年份:1998
- 资助金额:
$ 25.73万 - 项目类别:
INTEGRINS AND T CELL DEVELOPMENT AND FUNCTION
整合素与 T 细胞的发育和功能
- 批准号:
2650060 - 财政年份:1997
- 资助金额:
$ 25.73万 - 项目类别:
STRUCTURE/FUNCTION OF THE INTEGRIN ALPHA 1 BETA 1
整合素 ALPHA 1 BETA 1 的结构/功能
- 批准号:
6386004 - 财政年份:1990
- 资助金额:
$ 25.73万 - 项目类别:
STRUCTURE AND FUNCTION OF THE INTEGRIN ALPHA1 BETA1
整合素 ALPHA1 BETA1 的结构和功能
- 批准号:
3303765 - 财政年份:1990
- 资助金额:
$ 25.73万 - 项目类别:
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