GENE DELIVERY OF OP-1 IN RODENT MODEL OF RENAL FIBROSIS

OP-1 在啮齿动物肾纤维化模型中的基因递送

• 批准号: 2733945
• 负责人: GLENN A MCDONALD
• 金额: $ 1.58万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2733945
• 关键词: Adenoviridae; bone metabolism; cystic kidney; diabetic nephropathy; fibrosis; gene expression; gene therapy; genetic manipulation; genetic promoter element; histology; laboratory mouse; molecular cloning; nucleic acid sequence; osteogenesis; serology /serodiagnosis; transfection; transfection /expression vector; urinalysis

The two major goals of this research proposal are the following: to construct an optimal replication deficient adenoviral expression vector; and to evaluate the delivery of a therapeutic protein from this vector in a preclinical model of renal fibrosis. To this end I will utilize sophisticated molecular biological techniques to optimize the construct that I have made and make novel vectors as well. Protein expression will be evaluated in-vivo. After this evaluation is complete I will utilize this vector therapeutically in both a renal ablation and diabetic model of renal fibrosis.

这项研究建议的两个主要目标如下： 构建最佳复制缺陷型腺病毒表达载体 载体;以及评估治疗性蛋白质从 该载体在肾纤维化的临床前模型中。为此 我会利用先进的分子生物学技术 优化我所做的结构， 好.将在体内评估蛋白质表达。在此之后 评估完成后，我将使用这个载体治疗 在肾消融和糖尿病肾纤维化模型中。