DIETARY SOY ISOFLAVONES AND PROSTATE CANCER PROGRESSION

膳食大豆异黄酮与前列腺癌进展

• 批准号: 2712797
• 负责人: JIN-RONG ZHOU
• 金额: $ 3.05万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2712797
• 关键词: RNase protection assay; antineoplastics; athymic mouse; dietary supplements; fibroblasts; flavones; male; metalloendopeptidases; neoplasm /cancer nutrition therapy; neoplastic cell; neoplastic process; nutrition related tag; prostate neoplasms; protease inhibitor; soybeans; western blottings

The objective of this proposal is to investigate the preventive role of soy isoflavones in prostate cancer (PCA) progression and angiogenesis. The specific aims are: l) to study the effects of soy isoflavones on inhibiting expression of membrane-type metalloproteinase (MT-MMP) by PCA cells, fibroblasts, or endothelial cells (ECs) in vitro, and 2) to determine if diets containing soy protein isolate (SPI) or purified isoflavones inhibit prostate tumor progression, angiogenesis, and MT-MMP expression in vivo. Human PCA cells (PC-3, DU 145, and LNCAP), fibroblasts, and ECs will be treated with isoflavones. MMPs-containing conditioned media and MT-MMP-containing plasma membranes will be prepared. RNase protection assays, Western blotting, and zymography will be applied to determine mRNA, protein, and activity of MT-MMP to test the hypothesis that isoflavones inhibit PCA progression, and prostate tumor angiogenesis by inhibiting MT-MMP expression by PCA and fibroblasts, and ECs, respectively. In animal study, human PCA cell-inoculated nude mice will be fed control, SPI-based, or isoflavone-supplemented diets. Tumor sizes will be measured, and tumors removed for determinations of MT-MMP mRNA, protein and activity to evaluate the effects of isoflavones on in vivo inhibition of PCA progression. Anti-angiogenic effect of isoflavones will be evaluated by immunohistochemical analysis of microvessels.

这项建议的目的是调查……的预防作用

项目成果

Inhibition of orthotopic growth and metastasis of androgen-sensitive human prostate tumors in mice by bioactive soybean components.

生物活性大豆成分抑制小鼠体内雄激素敏感的人类前列腺肿瘤的原位生长和转移。

• DOI: 10.1002/pros.10141
• 发表时间: 2002
• 期刊: The Prostate
• 作者: Zhou,Jin-Rong;Yu,Lunyin;Zhong,Ying;Nassr,RenaL;Franke,AdrianA;Gaston,SandraM;Blackburn,GeorgeL
• 通讯作者: Blackburn,GeorgeL

Inhibition of murine bladder tumorigenesis by soy isoflavones via alterations in the cell cycle, apoptosis, and angiogenesis.

• DOI: 10.1016/s0022-5347(05)68638-7
• 发表时间: 1998-11
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Jin-Rong Zhou;P. Mukherjee;Eric Gugger;Toshihide Tanaka;G. Blackburn;S. Clinton

Soy phytochemicals and tea bioactive components synergistically inhibit androgen-sensitive human prostate tumors in mice.

大豆植物化学物质和茶生物活性成分可协同抑制小鼠体内雄激素敏感的人类前列腺肿瘤。

• DOI: 10.1093/jn/133.2.516
• 发表时间: 2003
• 期刊: The Journal of nutrition
• 作者: Zhou,Jin-Rong;Yu,Lunyin;Zhong,Ying;Blackburn,GeorgeL
• 通讯作者: Blackburn,GeorgeL