TOPOISOMERASE II AND TELOMERESE IN CANCER AND AGING

拓扑异构酶 II 和端粒在癌症和衰老中的作用

• 批准号: 2830532
• 负责人: MARK T MULLER
• 金额: $ 23.15万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-15 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2830532
• 关键词: DNA replication; DNA topoisomerases; aging; cell cycle; cell senescence; chemical cleavage; chromosome walking; eukaryote; intermolecular interaction; molecular site; neoplasm /cancer genetics; nucleic acid repetitive sequence; protein binding; telomere

Topoisomerases are important biochemical targets for chemotherapeutic intervention in a number of different cancers. These enzymes are important in many central genetic events of both normal and transformed cells. This proposal is based upon new findings that establish a link between type II topoisomerase and the physical ends of chromosomes (telomeres) in vivo. The interaction between topoisomerase II and telomeres represents a model system to explore the enzyme's recognition of biologically important DNA repeat elements that are widespread in the human genome. A comprehensive study of the endogenous interplay between eukaryotic topoisomerase II and telomeres is proposed to craft the convergence of these two areas to create new inroads for chemotherapeutic intervention in cancer and to integrate, these concepts with events related to cellular senescence and aging.

拓扑异构酶是化疗的重要生化靶点 干预多种不同的癌症。这些酶是 在正常和转化的许多中心遗传事件中都很重要 细胞。 该提案基于建立联系的新发现 II 型拓扑异构酶和染色体物理末端之间 （端粒）在体内。 拓扑异构酶 II 与拓扑异构酶 II 之间的相互作用 端粒代表了探索酶识别的模型系统 广泛存在于生物体中的具有重要生物学意义的DNA重复元件 人类基因组。 内源性相互作用的综合研究 提议利用真核拓扑异构酶 II 和端粒来制作 这两个领域的融合将创造新的突破 癌症的化疗干预并整合这些概念 与细胞衰老和衰老相关的事件。