DNA TOPOISOMERASES & LATE CELL CYCLE CHECKPOINTS

双（2，6-二氧代哌嗪），ICRF-193，是有效的非DNA损伤 拓扑异构酶II脱链活性的抑制剂。 的 抑制机制被解释为 ATP调节的蛋白钳模型。 ICRF-193抑制细胞分裂 但允许细胞周期穿越并进展为多倍体， 在G2检查站的延迟。 我们将使用传统的FCM方法， 检查细胞周期调节与各种细胞周期蛋白， 包括A和B1，荧光寿命分析将 在细胞结合的ICRF-123上进行，以分析其与 染色质

The bis(2,6-dioxopiperazine), ICRF-193, is potent non-DNA damaging inhibitor of the decantenation activity of topoisomerase II. The mechanism of inhibition is being interpreted in terms of an ATP-modulated protein-clamp model. ICRF-193 inhibits cell division but allows cell cycle traverse and progression to polyploidy with a delay at the G2 checkpoint. We will use conventional FCM methods to examine cell cycle regulation in conjunction with the various cyclins, including A and B1 and fluorescence lifetime analysis will be performed on cellular-bound ICRF-123 to analyze its interaction with chromatin.