NICOTINIC RECEPTORS AND SMOKING CESSATION

描述：（申请人摘要） 烟草制品使用成瘾是最常见和最常见的疾病之一。 破坏性的成瘾。 据估计，近一半 世界人口的一半吸烟。 很少有生物标记 通常用于监测戒烟提供长期信息 使用烟草制品，也没有任何类型的戒烟成功预测 吸烟的习惯。 我们已经证明了烟碱乙酰胆碱受体， 对香烟中尼古丁有反应的受体， 死后大脑的受试者谁是吸烟者在生活中，但下降到近 戒烟时间不同的受试者的对照值 在死亡之前。 尼古丁受体也在黑色素瘤的白色细胞中表达。 外周血，其在临床环境中容易获得。 外围 血液中的中性粒细胞对吸烟也有类似的调节反应 在大脑中看到的产品。 拟议的研究将开发测定方法， 外周血中烟碱受体水平作为 监测戒烟情况。 我们将1）优化烟碱受体 外周血中性粒细胞的结合测定，2）确定 不吸烟者和吸烟者血液中性粒细胞中烟碱受体的表达 有不同吸烟史的受试者，以及3）评估 这项试验是在实际的戒烟计划中进行的。

DESCRIPTION: (Applicant's Abstract) The addiction to use of tobacco products is one of the most common and most destructive addictions in the world today. It is estimated that nearly half of the world population smokes cigarettes. Few of the biological markers commonly used to monitor smoking cessation provide information on long term use of tobacco products, nor any type of prediction of success in quitting the smoking habit. We have shown that nicotinic acetylcholine receptors, the receptors responding to nicotine in cigarettes, are increased in postmortem brain of subjects who were smokers in life, but decline to near control values in subjects who had ceased to smoke for varying periods before death. Nicotinic receptors are also expressed in the white cells of peripheral blood, which is easy to obtain in a clinical setting. Peripheral blood neutrophils have a similar regulatory response to use of tobacco products as is seen in brain. The proposed studies would develop the assay for nicotinic receptor levels in peripheral blood as a biological marker for monitoring smoking cessation. We will 1) optimize the nicotinic receptor binding assay for peripheral blood neutrophils, 2) determine the range of nicotinic receptor expression in blood neutrophils in never-smokers and in subjects with variable smoking histories, and 3) evaluate the utility of this assay in an actual smoking cessation program.