NICOTINIC RECEPTORS AND SMOKING CESSATION

烟碱受体与戒烟

• 批准号: 6174839
• 负责人: SHERRY LEONARD
• 金额: $ 12.9万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6174839
• 关键词: bioassay; biomarker; bungarotoxins; clinical research; human subject; method development; neutrophil; nicotine; nicotinic receptors; receptor binding; receptor expression; receptor sensitivity; smoking cessation

DESCRIPTION: (Applicant's Abstract) The addiction to use of tobacco products is one of the most common and most destructive addictions in the world today. It is estimated that nearly half of the world population smokes cigarettes. Few of the biological markers commonly used to monitor smoking cessation provide information on long term use of tobacco products, nor any type of prediction of success in quitting the smoking habit. We have shown that nicotinic acetylcholine receptors, the receptors responding to nicotine in cigarettes, are increased in postmortem brain of subjects who were smokers in life, but decline to near control values in subjects who had ceased to smoke for varying periods before death. Nicotinic receptors are also expressed in the white cells of peripheral blood, which is easy to obtain in a clinical setting. Peripheral blood neutrophils have a similar regulatory response to use of tobacco products as is seen in brain. The proposed studies would develop the assay for nicotinic receptor levels in peripheral blood as a biological marker for monitoring smoking cessation. We will 1) optimize the nicotinic receptor binding assay for peripheral blood neutrophils, 2) determine the range of nicotinic receptor expression in blood neutrophils in never-smokers and in subjects with variable smoking histories, and 3) evaluate the utility of this assay in an actual smoking cessation program.

描述：（申请人摘要） 使用烟草制品成瘾是最常见和最严重的成瘾行为之一 当今世界的破坏性成瘾。 估计有近一半 世界人口吸烟。 生物标志物很少 通常用于监测戒烟情况，提供长期信息 使用烟草制品，也没有任何类型的戒烟成功预测 吸烟习惯。 我们已经证明烟碱乙酰胆碱受体， 对香烟中尼古丁作出反应的受体在 生前吸烟但吸烟率下降到接近吸烟者的受试者死后大脑 戒烟不同时期的受试者的控制值 临终前。 烟碱受体也在白细胞中表达 外周血，在临床环境中很容易获得。 周边 血液中性粒细胞对吸烟有类似的调节反应 在大脑中看到的产品。 拟议的研究将开发检测方法 外周血烟碱受体水平作为生物标志物 监测戒烟情况。 我们将1）优化烟碱受体 外周血中性粒细胞结合测定，2)确定范围 nicotinic receptor expression in blood neutrophils in never-smokers and in 具有不同吸烟史的受试者，以及3）评估 该测定在实际戒烟计划中进行。