GENOSENSOR DESIGN BASED ON FLEXURAL PLATE WAVE DEVICES

基于挠性板波器件的基因传感器设计

• 批准号: 2642265
• 负责人: LARRY J KEPLEY
• 金额: $ 10万
• 依托单位: TPL, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-22 至 2000-08-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2642265
• 关键词: biomedical equipment development; biosensor device; computer assisted sequence analysis; computer system design /evaluation; computer system hardware; genome; human genetic material tag; nucleic acid hybridization; nucleic acid probes

Current genosensor methods involve radioactive, fluorescence, or chemiluminescence techniques that are slow, relatively insensitive, and expensive compared to advanced microsensor methods. For rapid molecular diagnostics, novel dynamic sensors are needed that combine the speed, high sensitivity, and high specificity of DNA hybridization with the speed and ultrasensitivity of microelectronic transducers. In collaboration with the Molecular Diagnostics Core Laboratory of the University of New Mexico, TPL, Inc. will develop an innovative sensor based on microfabricated porous transducers containing short, immobilized oligonucleotides for direct quantitation of specific sequences (point mutations). The reusable sensor will incorporate flexural plate-wave (FPW) devices that have demonstrated extreme sensitivity to mass changes (pg/cnf), which theoretically allows detection of femtomole quantities. TPL, Inc. will design, fabricate and test FPW-based sensors, utilizing innovative microstructured materials, to determine their sensitivity and specificity for hybridized target DNA. TPL has extensive experience in the design and characterization of acoustic wave microsensors. Much of the electronics needed for FPW devices have been developed and integrated into commercial products, such as TPL's Thin Film Analyzer. By combining TPL's expertise in advanced sensors with MDCL/UNM's experience in molecular diagnostics, this program will develop a new generation of genosensor for clinical analysis. PROPOSED COMMERCIAL APPLICATIONS: A new ultrasensitive, highly specific genosensor which provides substantially improved detection speed would find broad use in the medical diagnostics, forensic science, and pharmaceutical industries. Furthermore, such a system would establish a technology basis for extension of FPW-sensors to DNA sequencing for basic and applied research applications, such as the Human Genome Project.

目前的基因传感器方法包括放射性、荧光或 化学发光技术，速度慢，相对不敏感， 与先进的微传感器方法相比，成本很高。用于快速分子 诊断学，需要新的动态传感器结合速度， DNA杂交法的高敏感性、高特异性 微电子换能器的速度和超灵敏。 与分子诊断学核心实验室合作 新墨西哥大学第三方物流公司将开发一种创新的传感器 基于含有短的、固定化的微孔换能器 用于直接定量特定序列的寡核苷酸(点 突变)。可重复使用的传感器将结合弯曲板波 (Fpw)对质量变化表现出极高敏感性的设备 (PG/CNF)，理论上允许检测飞摩尔量。 第三方物流公司将设计、制造和测试基于FPW的传感器，利用 创新的微结构材料，以确定其敏感性和 杂交靶DNA的特异性。 TPL在设计和表征方面拥有丰富的经验 声波微型传感器。FPW所需的大部分电子设备 设备已经被开发并集成到商业产品中，例如 作为第三方物流的薄膜分析仪。通过结合第三方物流在高级领域的专业知识 具有MdCL/UNM分子诊断经验的传感器，此程序 将开发用于临床分析的新一代基因传感器。 建议的商业应用： 一种新型的超灵敏、高特异度的基因传感器 大大提高的检测速度将在 医疗诊断学、法医学和制药业。 此外，这种系统将为以下方面奠定技术基础 光纤传感器在DNA测序基础和应用研究中的应用 应用，如人类基因组计划。