NEW RNA IN SITU HYBRIDIZATIONS WITH HIGH RESOLUTION

描述(改编自《调查者摘要》)：很好 已知某些基因(myc， RAS)，包括癌基因的激活，或一些病毒感染，可以 会导致癌症的发展。建议的主要目标是 研究目的是开发一种新的、快速、超灵敏、安全的检测方法 检测病毒或细菌感染或基因变化 通过RNA或DNA原位杂交方法进行表达 亚细胞水平的定位。方法是使用 等离子体激元共振粒子的非凡能力，它可以 提供无毒、非常明亮、坚固、彩色标记的探测器，可以 与任何感兴趣的经修饰的核酸探针结合。这是一项新的 与记者制度相比，记者制度应该有重大优势 当前使用的基于放射性或非放射性的检测系统 商业上可以买到的。这项研究的最初目标将是 开发特定的试剂、方案和相关技术 将是用户友好的，并且对于大范围的研究和 临床应用，以及将 允许对临床样本进行机器人和自动化分析。 建议的商业应用： 商业应用将是提取特定的试剂， 协议和相关技术，这些技术是在 研究计划，并安排合适的诊断试剂盒和 待售的仪器。

DESCRIPTION (Adapted from the Investigator's Abstract): It is well known that some alterations in transcription of certain genes (myc, ras), including activation of oncogenes, or some viral infections, can lead to the development of cancer. The major goal of the proposed research is to develop a new, rapid, ultra-sensitive, safe method of detecting viral or bacterial infection, or alterations in gene expression, by RNA or DNA in situ hybridization methodology with localization at the subcellular level. The approach is to use the extraordinary capabilities of plasmon resonant particles which can provide non-toxic, very bright, robust, color-labeled probes that can be bound to any modified nucleic acid probe of interest. This new reporter system should have significant advantages compared with the currently used radioactive or non-radioactive based detection systems commercially available. The initial aims of the research will be to develop the specific reagents, protocols, and associated technology that will be user friendly, and affordable for wide scale research and clinical applications, and the instrumentation and software that will allow for robotic and automated analysis of clinical samples. PROPOSED COMMERCIAL APPLICATION: The commercial applications will be to take the specific reagents, protocols, and associated technology that is developed under the Research Program, and arrange for suitable Diagnostic Kits and Instrumentation to be offered for sale.