NEW TECHNIQUE FOR HIGH RESOLUTION MAPPING OF DNA

本项目的主要目标是开发一种新的可靠、高效的 高分辨率、高效率、高性价比的技术 检测人类基因组和部分片段中的遗传缺陷 关于DNA的。我们的方法是利用 等离子激元共振粒子可以提供无毒，非常明亮， 坚固的彩色标记探针可以结合到任何所需的特定- 基因座DNA片段，然后与目的DNA链杂交。 我们希望在标记探针之间实现更低的空间分辨率 那0.5KB。我们将开发特定的试剂、方案和 相关技术将是用户友好的，并且经济实惠 广泛的临床应用。特殊的应用包括DNA 芯片阵列。 建议的商业应用： 既有大规模的商业应用，也有小型的商业应用。论 即刻小规模、有无的制造和销售 观察等离子体共振所需的特殊显微镜附件 粒子，以及探针包裹的等离子体激元共振粒子的销售， (PRP)和相关试剂。在大范围内，有一个应用程序 将PRP技术应用于基因缺陷的医学诊断测试， 免疫分析和免疫细胞学。机器人版本是可能的。

The major goal of this project is to develop a new reliable, high resolution, efficient, and cost effective technique for the general detection of genetic defects in the human genome, and in selected pieces of DNA. The approach is to utilize the extraordinary capabilities of plasmon resonant particles which can provide non-toxic, very bright, robust, color-labeled probes that can be bound to any desired specific- locus DNA fragment, and then hybridized to the DNA strand of interest. We expect to achieve a spatial resolution between marker probes of less that 0.5 KB. We shall develop the specific reagents, protocols, and associated technology which will be user-friendly, and affordable for wide scale clinical applications. Particular applications include DNA chip arrays. PROPOSED COMMERCIAL APPLICATIONS: There are both large and small scale commercial applications. On the immediate small scale, there if the manufacture and sale of the specialized microscope accessories needed to observe the Plasmon Resonant Particles, and the sale of the probe-coated Plasmon Resonant Particles, (PRP) and related reagents. On the large scale, there is the application of the PRP techniques to medical diagnostic tests for gene defects, immunoassays, and immunocytology. A robotic version is possible.