NEW TECHNIQUE FOR HIGH RESOLUTION MAPPING OF DNA

描述：（改编自申请人摘要）。 的主要目标 本项目旨在开发一种新的可靠，高分辨率，高效， 用于遗传缺陷的一般检测的成本有效的技术 在人类基因组中，在选择的DNA片段中。 采取方法是 利用等离子体共振粒子的非凡能力 其可以提供无毒的、非常明亮的、稳健的、颜色标记的探针 可以与任何所需的特异性基因座DNA片段结合，然后 与感兴趣的DNA链杂交。 调查人员预计， 在标记探针之间实现小于0.5KB的空间分辨率。 研究者应制定具体的试剂、方案， 相关的技术，这将是用户友好，负担得起的， 广泛的临床应用。

DESCRIPTION: (Adapted from the Applicant's Abstract). The major goal of this project is to develop a new reliable, high resolution, efficient, and cost effective technique for the general detection of genetic defects in the human genome, and in selected pieces of DNA. The approach is to utilize the extraordinary capabilities of plasmon resonant particles which can provide non-toxic, very bright, robust, color-labeled probes that can be bound to any desired specific-locus DNA fragment, and then hybridized to the DNA strand of interest. The investigators expect to achieve a spatial resolution between marker probes of less than 0.5 KB. The investigators shall develop the specific reagents, protocols, and associated technology which will be user- friendly, and affordable for wide scale clinical applications.