AMINO ACID ANALYSIS USING MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY
使用胶束电动毛细管色谱法进行氨基酸分析
基本信息
- 批准号:6239888
- 负责人:
- 金额:$ 8.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-08-01 至 1998-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The trace analysis of biological components has been a critical key
towards the development of modern biochemistry and medicine. One
particularly powerful contribution has been the development of
separations techniques capable of performing rapid and quantitative
analysis on small amounts of biogenic materials. High performance liquid
chromatography (HPLC) has proven capable of resolving all naturally
occurring amino acids in a relatively short time frame providing a robust
method for the determination of amino acid composition of proteins and
peptides. One significant drawback to HPLC, as well as many other
currently available analysis methods, is the quantity of biological
sample required to perform the analysis since heroic efforts are often
required to isolate the components(s) of interest. As a conseuence, more
sensitive microanalysis separation schemes are being developed to provide
increased mass sensitivities and improved resolving capabilities. Among
these new techniques, capillary electrophoresis (CE) has proved to be an
exceptionally capable and cost effective tool.
The overall objective of this project is to extend the utility of
micellar electrokinetic capillary chromatography (MECC), a mode of CE,
towards the microanalysis of biological materials. The proposed studies
will evaluate a novel series of organic additives used to promote changes
in the electrochromatographic selectivity towards biogenic molecules in
micellar systems at low concentrations. More specifically, the aim is
to provide a rapid and reproducible separation scheme that produces
complete resolution of all naturally occurring amino-acids with femto-
mole mass sensitivities. This objective will be approached by evaluating
a novel series of organic additives combined with traditional micellar
phases to determine their effects on electrochromatographic retention and
selectivity through a series of systematic studies altering each
chromatographic parameter independently. Upon a firm understanding of
the behavior of these additives in micellar systems, optimization of a
MECC method for the quantitative determination of derivatized amino acids
will be addressed. This project will provide a better understanding of
the utility of modified micellar phases in MECC towards biogenic species
and if successful provide a cost effective alternative to microanalysis
of amino acids.
生物成分的痕量分析一直是生物学研究的关键
现代生物化学和医学的发展。 一
特别有力的贡献是,
分离技术能够进行快速和定量
分析少量的生物材料。 高效液相
高效液相色谱法(HPLC)已被证明能够解决所有自然
在相对较短的时间范围内产生氨基酸,
测定蛋白质氨基酸组成的方法
缩氨酸 HPLC的一个显著缺点,以及许多其他缺点,
目前可用的分析方法,是生物量
由于英勇的努力往往是
需要分离感兴趣的组分。 作为一个连接,更多
正在开发灵敏的微量分析分离方案,
提高了质量灵敏度并改进了分辨能力。 之间
这些新技术,毛细管电泳(CE)已被证明是一个
非常有能力和成本效益的工具。
该项目的总体目标是扩大
胶束电动毛细管色谱(MECC),CE的一种模式,
对生物材料的微量分析。 拟议的研究
将评估一系列用于促进变革的新型有机添加剂
在对生物分子的电色谱选择性中,
低浓度的胶束体系。 更具体地说,目的是
为了提供快速且可再现的分离方案,
所有天然存在的氨基酸与毫微微-
摩尔质量灵敏度 这一目标将通过评估
一系列新型有机添加剂与传统的胶束相结合,
相,以确定其对电色谱保留的影响,
通过一系列系统的研究,
色谱参数独立。 基于对
这些添加剂在胶束体系中的行为,
MECC法定量测定衍生化氨基酸
将得到解决。 该项目将提供一个更好的了解
改性胶束相在MECC中对生物物种的应用
如果成功的话,提供了一种成本有效的替代微量分析的方法,
的氨基酸。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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JOEL T SMITH其他文献
JOEL T SMITH的其他文献
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{{ truncateString('JOEL T SMITH', 18)}}的其他基金
EXPLORATIONS OF CAPILLARY ELECTROPHORESIS IN THE ANALYSIS OF MODEL GLYOPROTEINS
毛细管电泳在模型糖蛋白分析中的探索
- 批准号:
8167538 - 财政年份:2010
- 资助金额:
$ 8.35万 - 项目类别:
EQUIPMENT FOR SOUTHEASTERN OKLAHOMA STATE UNIVERSITY
东南俄克拉荷马州立大学设备
- 批准号:
7960020 - 财政年份:2009
- 资助金额:
$ 8.35万 - 项目类别:
EQUIPMENT FOR SOUTHEASTERN OKLAHOMA STATE UNIVERSITY
东南俄克拉荷马州立大学设备
- 批准号:
7725098 - 财政年份:2008
- 资助金额:
$ 8.35万 - 项目类别:
EQUIPMENT FOR SOUTHEASTERN OKLAHOMA STATE UNIVERSITY
东南俄克拉荷马州立大学设备
- 批准号:
7610278 - 财政年份:2007
- 资助金额:
$ 8.35万 - 项目类别:
EQUIPMENT FOR SOUTHEASTERN OKLAHOMA STATE UNIVERSITY
东南俄克拉荷马州立大学设备
- 批准号:
7381662 - 财政年份:2006
- 资助金额:
$ 8.35万 - 项目类别: